College of Public Health, University of South China, Hengyang 421001, PR China.
Anal Chim Acta. 2011 Apr 1;690(2):234-9. doi: 10.1016/j.aca.2011.02.030. Epub 2011 Feb 18.
A novel method for the determination of trace formaldehyde in blood plasma has been established by using resonance fluorimetry technique. It was based on the fact that oxidation of pyronine Y by potassium bromate was catalyzed by formaldehyde in sulfuric acid. When the wavelength interval was at Δλ=0 nm, it was found that the decreased intensity (ΔF) of resonance fluorescence at 574.6 nm was proportional to the concentration of formaldehyde in the range of 1.27×10(-2) to 2.28 μg mL(-1). The limit of detection and the average recovery for formaldehyde were 3.80 ng mL(-1) and 101.6% (n=6), respectively. The present method had been applied to the determination of trace formaldehyde in blood plasma, and the obtained results were in good agreement with those obtained by the resonance light scattering method.
建立了一种用共振荧光法测定血浆中痕量甲醛的新方法。其原理是甲醛在硫酸中能催化高碘酸钾氧化吡啰红 Y。当波长间隔为Δλ=0nm 时，发现在 574.6nm 处共振荧光的强度（ΔF）的降低与甲醛的浓度在 1.27×10(-2)到 2.28μg·mL(-1)范围内成正比。甲醛的检出限和平均回收率分别为 3.80ng·mL(-1)和 101.6%（n=6）。该方法已用于测定血浆中痕量甲醛，所得结果与共振光散射法一致。
