Gaziantep University, Faculty of Arts and Sciences, Department of Biology, Gaziantep 27310, Turkey.
Chemosphere. 2011 Jun;84(1):39-46. doi: 10.1016/j.chemosphere.2011.02.087. Epub 2011 Mar 23.
The present study was conducted in order to investigate pro-oxidant activity of dimethoate in liver and brain tissues following sublethal pesticide exposure for 5, 15 and 30 d by using SOD, GPx, CAT enzyme activities and lipid peroxidation as biomarkers as well as DNA damaging potential via detecting% Tail DNA, Tail moment and Olive tail moment as endpoints in erythrocytes of Oncorhynchus mykiss in an in vitro experiment. Antioxidant enzyme activities were found to elicit two staged response which was an initial induction followed by a sharp inhibition in liver tissue while a sustained increase in GPx activity and slight stimulation in SOD activity were detected in brain tissue. Lipid peroxidation showed an ascending pattern throughout the exposure period in both tissues and a decreasing trend was determined in tissue protein levels which was proved to be positively correlated with duration. Similar findings were obtained from outcomes preferred to quantify DNA damage and TM was decided to reflect the extent of damage more sensitively because of determined positive correlation with concentrations applied. Considering these results, it can be concluded that oxidative stress condition evoked by dimethoate could not be responded effectively and genotoxic nature of pesticide was proven by determined clastogenic effect possibly via being an alkylation agent or stimulating the production of reactive species.
本研究旨在通过使用 SOD、GPx、CAT 酶活性和脂质过氧化作为生物标志物，以及通过检测红细胞中的 %尾 DNA、尾矩和橄榄尾矩作为终点，研究亚致死剂量农药暴露 5、15 和 30 天后对鱼肝和脑组织的促氧化剂活性。研究发现，抗氧化酶活性呈现出两阶段反应，先是在肝组织中诱导，然后急剧抑制，而在脑组织中则检测到 GPx 活性持续增加和 SOD 活性略有刺激。在两个组织中，脂质过氧化在整个暴露期间呈现上升趋势，而组织蛋白水平呈下降趋势，这被证明与持续时间呈正相关。从更倾向于量化 DNA 损伤的结果中获得了类似的发现，并且由于与应用浓度呈正相关，因此决定 TM 更敏感地反映损伤程度。考虑到这些结果，可以得出结论，二甲氧基引起的氧化应激状态可能无法得到有效应对，并且农药的遗传毒性已通过确定的断裂剂作用或通过刺激活性物质的产生得到证明。
