Department of Pathology, Clinical Center of the University of Sarajevo, Bolnička 25, BA-71000, Sarajevo, Bosnia and Herzegovina.
Virchows Arch. 2011 Oct;459(4):377-82. doi: 10.1007/s00428-011-1144-4. Epub 2011 Sep 4.
We compared microvascular density (MVD), lymph vessel density (LVD), and the expression of hypoxia pathway-associated proteins between primary triple-negative adenoid cystic carcinoma of the breast (TN-ACC) and grade-matched triple-negative breast carcinomas of no special type (TNBC). Twelve TN-ACC and 15 TNBC were investigated immunohistochemically for CD31, podoplanin (D2-40), von Hippel-Lindau protein (pVHL), and hypoxia-inducible factor-1alpha (HIF-1α) protein. All cases were lymph node negative (pN0). The study revealed a median MVD (CD31) of 34 vessels/mm(2) (mean ± SD, 41.33 ± 6.5/mm(2)) in the TN-ACC subgroup and a median of 55 microvessels (mean ± SD, 54.9 ± 6.3/mm(2)) in the TNBC subgroup. The median LVD (D2-40) was 10.5/mm(2) (mean ± SD, 11.9 ± 1.5/mm(2)) in the TN-ACC subgroup and 15.0/mm(2) (mean ± SD, 16.9 ± 2.5/mm(2)) lymph vessels in the TNBC subgroup. The differences were not statistically significant (P = 0.93, P = 0.67, respectively). pVHL was detectable in all TN-ACCs whereas two cases of TNBC had less than 5% of the positive cells. HIF-1α protein expression was significantly higher in the tumor cell population than in adjacent normal cells in both subgroups (P = 0.009 for TNBC and P = 0.028 for TN-ACC, respectively), but there was no significant difference between the two tumor groups. Up-regulation of the hypoxia-induced signaling is seen in both TN-ACC and grade-matched TNBC. Despite its perceived low malignant potential, TN-ACC of the breast does not differ in the number of blood and lymphatic vessels in comparison with the grade-matched TNBC. The reported biologic differences between TN-ACC and TNBC do not appear to result from neoangiogenesis.
我们比较了原发性乳腺三阴性腺样囊性癌（TN-ACC）和分级匹配的三阴性乳腺癌（TNBC）之间的微血管密度（MVD）、淋巴管密度（LVD）和缺氧途径相关蛋白的表达。12 例 TN-ACC 和 15 例 TNBC 行 CD31、podoplanin（D2-40）、von Hippel-Lindau 蛋白（pVHL）和缺氧诱导因子-1α（HIF-1α）蛋白免疫组化检测。所有病例均为淋巴结阴性（pN0）。研究显示，TN-ACC 亚组中 CD31 平均微血管密度（MVD）中位数为 34 个血管/平方毫米（34 个/平方毫米）（中位数±SD，41.33±6.5/平方毫米），而 TNBC 亚组中 CD31 平均微血管密度（MVD）中位数为 55 个血管/平方毫米（55 个/平方毫米）（中位数±SD，54.9±6.3/平方毫米）。TN-ACC 亚组的平均淋巴管密度（LVD）中位数为 10.5 个/平方毫米（10.5 个/平方毫米）（中位数±SD，11.9±1.5/平方毫米），而 TNBC 亚组的 LVD 中位数为 15.0 个/平方毫米（15.0 个/平方毫米）（中位数±SD，16.9±2.5/平方毫米）。差异无统计学意义（P=0.93，P=0.67）。所有 TN-ACC 均检测到 pVHL，而 2 例 TNBC 的阳性细胞比例均<5%。在两个亚组中，肿瘤细胞群中的 HIF-1α 蛋白表达均显著高于邻近正常细胞（P=0.009 用于 TNBC，P=0.028 用于 TN-ACC），但两组肿瘤之间无显著差异。在 TN-ACC 和分级匹配的 TNBC 中均可见缺氧诱导信号的上调。尽管 TN-ACC 的恶性程度较低，但与分级匹配的 TNBC 相比，其血管和淋巴管数量并无差异。TN-ACC 和 TNBC 之间报道的生物学差异似乎并非源于新生血管形成。
