Fortes P, Salvador G, Cônsoli F L
Depto de Entomologia e Acarologia, ESALQ, USP, Piracicaba, SP, Brasil.
Neotrop Entomol. 2011 Jan-Feb;40(1):89-96. doi: 10.1590/s1519-566x2011000100013.
Ovary development and maturation of Nezara viridula (L.) were evaluated by examining ovariole morphology and the alterations in the biochemical (protein synthesis related to reproduction) composition of the hemolymph. Quantitative and qualitative protein analyses were performed and ovary structural alterations for the pre-reproductive and reproductive stages were recorded. Total concentration of proteins in female hemolymph gradually increased until the end of the pre-mating stage, remaining unaltered thereafter. Proteins linked to reproduction (vitellogenins) appeared in the hemolymph 10 days after adult emergence and indicated the end of the pre-mating stage. After mating, total protein concentration in the hemolymph was lower compared to virgin females; vitellogenin levels were similar during most of the observation period. Oocyte development and maturation were gradual and age dependent. Ten-day-old females had chorionated oocytes ready for fertilization. Mating did not stimulate oocyte development in N. viridula, but the lack of mating activity appeared to have stimulated oocyte resorption in 17-day-old females.
通过检查卵巢小管形态以及血淋巴生化成分（与繁殖相关的蛋白质合成）的变化，对绿盲蝽（Nezara viridula (L.)）的卵巢发育和成熟情况进行了评估。进行了蛋白质的定量和定性分析，并记录了生殖前期和生殖期的卵巢结构变化。雌性血淋巴中蛋白质的总浓度在交配前期结束前逐渐增加，此后保持不变。与繁殖相关的蛋白质（卵黄原蛋白）在成虫羽化10天后出现在血淋巴中，这表明交配前期结束。交配后，血淋巴中的总蛋白浓度低于未交配的雌性；在大多数观察期内，卵黄原蛋白水平相似。卵母细胞的发育和成熟是渐进的且与年龄有关。10日龄的雌性有已形成卵壳的卵母细胞，准备好进行受精。交配并未刺激绿盲蝽的卵母细胞发育，但缺乏交配活动似乎刺激了17日龄雌性的卵母细胞吸收。
