USDA-ARS National Biological Control Laboratory, Stoneville, MS.
Resigned from the USDA-ARS National Biological Control Laboratory, Stoneville, MS.
J Insect Sci. 2020 Aug 1;20(5). doi: 10.1093/jisesa/ieaa092.
The southern green stink bug (SGSB), Nezara viridula (L.), is an important agricultural pest in the United States. Limited information is available on the morphology of the female's reproductive system in relation to morphological changes associated with the number of eggs produced and egg masses oviposited. The ability to assess reproductive health and reproductive status based on ovarian morphology (i.e., physiological age-grading) can be an important tool for evaluating field populations and laboratory colonies intended for the application of different management strategies and experimental trials. Thus, the goal of this study was to develop a physiological age-grading system for SGSB. Females aged from 0 to 79 d chronologically randomly selected from laboratory colonies and dissected to assess ovarian morphology. Specific morphological differences in ovarian structures including differentiation of the ovarioles, deposition of yolk in the most proximal follicle, quantity and appearance of follicular relics, expansion of the lateral oviducts, and number of developing follicles per ovariole were related to chronological age, the number of eggs produced and number of egg masses. Based on specific combinations of these morphological characteristics, the continuum of ovarian development was divided into three nulliparous (i.e., 'no eggs'; N1, N2, and N3) and three parous stages (i.e., 'with eggs'; P1, P2, and P3). Direct relationships were noted between number of eggs produced and physiological age with over 7-fold higher number of eggs and 14-fold higher number of egg masses associated with the P2 and P3 stages, respectively.
南方绿椿象（SGSB），又称美洲芫菁，是美国一种重要的农业害虫。关于其雌性生殖系统的形态与产卵数量和卵块产卵管相关的形态变化的信息有限。基于卵巢形态评估生殖健康和生殖状态的能力（即生理年龄分级）可以是评估田间种群和实验室种群的重要工具，这些种群可用于应用不同的管理策略和实验试验。因此，本研究的目的是开发南方绿椿象的生理年龄分级系统。从实验室群体中随机选择 0 至 79 天龄的雌性个体进行解剖，以评估卵巢形态。卵巢结构的特定形态差异，包括卵原管的分化、最接近的滤泡中卵黄的沉积、滤泡遗迹的数量和外观、侧输卵管的扩张以及每个卵巢管中发育滤泡的数量，与年龄、产卵数量和卵块数量有关。基于这些形态特征的特定组合，卵巢发育的连续性分为三个未产卵阶段（即“无卵”；N1、N2 和 N3）和三个产卵阶段（即“有卵”；P1、P2 和 P3）。研究发现，产卵数量和生理年龄之间存在直接关系，与 N1、N2 和 N3 阶段相比，P2 和 P3 阶段的产卵数量分别高出 7 倍和 14 倍，而卵块数量分别高出 14 倍和 28 倍。
