Micro-Phage Laboratory, Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA.
Department of Veterans Affairs, Salt Lake City Health Care System, Salt Lake City, UT 84148, USA.
Viruses. 2023 Feb 7;15(2):460. doi: 10.3390/v15020460.
causes the majority of implant-related infections. These infections present as biofilms, in which bacteria adhere to the surface of foreign materials and form robust communities that are resilient to the human immune system and antibiotic drugs. The heavy use of broad-spectrum antibiotics against these pathogens disturbs the host's microbiome and contributes to the growing problem of antibiotic-resistant infections. The use of bacteriophages as antibacterial agents is a potential alternative therapy. In this study, bioluminescent strains of S. aureus were grown to form 48-h biofilms on polyether ether ketone (PEEK), a material used to manufacture orthopaedic implants, in either static or dynamic growth conditions. Biofilms were treated with vancomycin, staphylococcal phage, or a combination of the two. We showed that vancomycin and staph phages were able to independently reduce the total bacterial load. Most phage-antibiotic combinations produced greater log reductions in surviving bacteria compared to single-agent treatments, suggesting antimicrobial synergism. In addition to demonstrating the efficacy of combining vancomycin and staph phage, our results demonstrate the importance of growth conditions in phage-antibiotic combination studies. Dynamic biofilms were found to have a substantial impact on apparent treatment efficacy, as they were more resilient to combination treatments than static biofilms.
导致了大多数植入物相关感染。这些感染表现为生物膜，其中细菌附着在异物表面并形成坚固的群落，对人体免疫系统和抗生素药物具有很强的抵抗力。广泛使用广谱抗生素来对抗这些病原体，扰乱了宿主的微生物组，并导致抗生素耐药性感染问题日益严重。噬菌体作为抗菌剂的使用是一种潜在的替代治疗方法。在这项研究中，金黄色葡萄球菌的生物发光菌株在聚醚醚酮（PEEK）上生长，形成 48 小时生物膜，PEEK 是用于制造矫形植入物的材料，在静态或动态生长条件下进行。生物膜用万古霉素、葡萄球菌噬菌体或两者的组合进行处理。我们表明，万古霉素和葡萄球菌噬菌体能够独立地减少总细菌负荷。与单一药物治疗相比，大多数噬菌体-抗生素组合产生了更大的对数减少，表明存在抗菌协同作用。除了证明将万古霉素和葡萄球菌噬菌体结合使用的功效外，我们的结果还表明生长条件在噬菌体-抗生素联合研究中的重要性。动态生物膜对明显的治疗效果有重大影响，因为它们比静态生物膜更能抵抗联合治疗。
