College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, People's Republic of China.
Phytochem Anal. 2011 Jul-Aug;22(4):313-21. doi: 10.1002/pca.1282. Epub 2011 Mar 25.
Radix Saposhnikoviae is one of the most famous Chinese herbal medicines with many pharmacological activities towards inflammatory symptoms and antioxidation. Chromones are considered as one of the effective components. It is important to find a reasonable method to extract the chromones in S. divaricata.
To develop an ultrasonic-assisted extraction (UAE) to extract chromones in Radix Saposhnikoviae and to optimise extraction conditions.
Four chromones (prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside and sec-O-glucosylhamaudol) were extracted by the UAE method combined with response surface methodology (RSM). Box-Behnken design (BBD) was applied to evaluate the effects of three independent variables (ethanol concentration, extraction time and extraction temperature) on the chromones yield of Radix Saposhnikoviae.
Correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be employed to optimise the extraction of chromones by UAE method. The optimal conditions to obtain the highest chromones yield of Radix Saposhnikoviae were a solvent of 75% ethanol, an extraction time of 48 min and an extraction temperature of 67°C.
Under these optimal conditions, the experimental values agreed closely with the predicted values. The analysis of variance indicated a high goodness of model fit and the success of RSM method for optimising chromones extraction in Radix Saposhnikoviae.
防风是一种具有多种抗炎和抗氧化作用的著名中草药，其中类黄酮被认为是其主要活性成分之一。因此，找到一种合理的方法来提取防风中的类黄酮非常重要。
采用超声辅助提取（UAE）法提取防风中的类黄酮，并对提取条件进行优化。
采用响应面法（RSM）结合超声辅助提取法（UAE）提取防风中的 4 种类黄酮（原-O-葡萄糖基升麻素、升麻素、5-O-甲基维斯阿米醇苷和 sec-O-葡萄糖基哈马醇）。采用 Box-Behnken 设计（BBD）考察乙醇浓度、提取时间和提取温度 3 个独立变量对防风中类黄酮提取率的影响。
对数学回归模型的相关性分析表明，二次多项式模型可用于优化 UAE 法提取类黄酮。获得防风中类黄酮最大提取率的最佳条件为：溶剂为 75%乙醇，提取时间 48 分钟，提取温度 67°C。
在这些最佳条件下，实验值与预测值吻合较好。方差分析表明，模型拟合度良好，RSM 方法成功优化了防风中类黄酮的提取。
