Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
DNA Cell Biol. 2011 Jul;30(7):461-8. doi: 10.1089/dna.2010.1168. Epub 2011 Mar 27.
Adipose triglyceride lipase (ATGL) is a crucial lipase that catalyzes triglyceride hydrolysis. In this study, reverse transcription (RT)-polymerase chain reaction, rapid amplification of cDNA ends, and mRNA real-time analysis were performed to characterize the ATGL gene in zebra finch and Java sparrow. Two cDNA of the zebra finch ATGL (zfATGL) gene were 1877 and 1626 bp were obtained; both encoded the same 481 amino acid protein. A 251 bp indel in the 3' UTR led to two variant transcripts. The zfATGL gene at chromosome 5 spanned over 28 Kb in length and comprised 9 exons. As in chicken, both the zfATGL gene and its intron 1 were much longer than mammals, which seemed to be one characteristic of ATGL gene in birds. The zfATGL gene was predominantly expressed in abdominal fat, subcutaneous fat, breast muscle, and leg muscle in both male and female. Two cDNA (1938 and 1885 bp) of the Java sparrow ATGL gene were also obtained, both of which encoded the same 481 amino acid protein. A 53 bp indel in 3' UTR led to two variant transcripts. The zfATGL gene was predominantly expressed in breast muscle, subcutaneous fat, abdominal fat, heart, and leg muscle. ATGL homology analysis showed that GASAG and GCGFLG motifs in the patatin region were conserved among 16 species. It was further indicated that the 251- and 53 bp indels at the 3' UTR of ATGL were potential binding sites for miRNA tgu-miR-2987. In conclusion, our study identified the full length cDNA and their variant transcripts of zfATGL and Java sparrow ATGL, and they were predominantly expressed in muscle, fat, and heart tissues.
脂肪甘油三酯脂肪酶 (ATGL) 是一种关键的脂肪酶，可催化甘油三酯水解。在这项研究中，我们通过反转录（RT）-聚合酶链反应、快速扩增 cDNA 末端和 mRNA 实时分析来研究斑马雀和 Java 麻雀的 ATGL 基因。我们获得了两个斑马雀 ATGL（zfATGL）基因的 cDNA，长度分别为 1877bp 和 1626bp，它们都编码相同的 481 个氨基酸的蛋白质。在 3'UTR 中有一个 251bp 的插入/缺失导致了两种变体转录本。位于第 5 号染色体上的 zfATGL 基因长度超过 28kb，包含 9 个外显子。与鸡一样，zfATGL 基因及其内含子 1都比哺乳动物长得多，这似乎是鸟类 ATGL 基因的一个特征。zfATGL 基因在雄性和雌性的腹部脂肪、皮下脂肪、胸肌和腿肌中都有强烈表达。我们还获得了 Java 麻雀 ATGL 基因的两个 cDNA（1938bp 和 1885bp），它们都编码相同的 481 个氨基酸的蛋白质。在 3'UTR 中有一个 53bp 的插入/缺失导致了两种变体转录本。zfATGL 基因在胸肌、皮下脂肪、腹部脂肪、心脏和腿肌中都有强烈表达。ATGL 同源性分析表明，在 16 个物种的 patatin 区域中，GASAG 和 GCGFLG 基序是保守的。这进一步表明，ATGL 3'UTR 中的 251bp 和 53bp 插入/缺失可能是 miRNA tgu-miR-2987 的结合位点。总之，我们的研究鉴定了 zfATGL 和 Java 麻雀 ATGL 的全长 cDNA 及其变体转录本，它们主要在肌肉、脂肪和心脏组织中表达。
