加拿大野鸭中低致病性禽流感病毒的检测：两种采样方法的比较。

Detection of low pathogenic avian influenza viruses in wild ducks from Canada: comparison of two sampling methods.

作者信息

Parmley E Jane, Soos Catherine, Breault André, Fortin Madeleine, Jenkins Emily, Kibenge Fred, King Robin, McAloney Keith, Pasick John, Pryor Sydney Paul, Robinson John, Rodrigue Jean, Leighton Frederick A

机构信息

Canadian Cooperative Wildlife Health Centre, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.

出版信息

J Wildl Dis. 2011 Apr;47(2):466-70. doi: 10.7589/0090-3558-47.2.466.

DOI:10.7589/0090-3558-47.2.466

PMID:21441201

Abstract

Surveillance for avian influenza viruses in wild birds was initiated in Canada in 2005. In 2006, in order to maximize detection of highly pathogenic avian influenza viruses, the sampling protocol used in Canada's Inter-agency Wild Bird Influenza Survey was changed. Instead of collecting a single cloacal swab, as previously done in 2005, cloacal and oropharyngeal swabs were combined in a single vial at collection. In order to compare the two sampling methods, duplicate samples were collected from 798 wild dabbling ducks (tribe Anatini) in Canada between 24 July and 7 September 2006. Low pathogenic avian influenza viruses were detected significantly more often (P<0.0001) in combined oropharyngeal and cloacal samples (261/798, 33%) than in cloacal swabs alone (205/798, 26%). Compared to traditional single cloacal samples, combined samples improved virus detection at minimal additional cost.

摘要

2005年，加拿大开始对野生鸟类中的禽流感病毒进行监测。2006年，为了最大程度地检测高致病性禽流感病毒，加拿大跨部门野生鸟类流感调查所采用的采样方案发生了变化。与2005年之前仅采集一份泄殖腔拭子不同，在采集时将泄殖腔拭子和咽拭子放在同一个小瓶中。为比较这两种采样方法，2006年7月24日至9月7日期间从加拿大798只野生水鸭（鸭族）中采集了重复样本。口咽和泄殖腔联合样本中检测到低致病性禽流感病毒的频率（261/798，33%）显著高于单独的泄殖腔拭子（205/798，26%）（P<0.0001）。与传统的单一泄殖腔样本相比，联合样本以最低的额外成本提高了病毒检测率。