State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):384-389. doi: 10.1099/ijs.0.030932-0. Epub 2011 Mar 25.
A gram-positive, aerobic, non-spore-forming actinobacterial strain, designated strain TL1(T), was isolated from pig manure in Wuhan, China. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H(4)). The major fatty acids (>10 %) were iso-C(16 : 0), iso-C(15 : 0) and C(17 : 1)ω8c. The genomic DNA G+C content was 70.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TL1(T) was most closely related to the type strains of Knoellia sinensis (98.5 %), Knoellia subterranea (98.2 %) and Knoellia aerolata (96.9 %). DNA-DNA relatedness values of strain TL1(T) with the type strains of K. sinensis and K. subterranea were 27.3 and 34.0 %, respectively. Comparison of phenotypic, chemotaxonomic and phylogenetic characteristics among strain TL1(T) and related organisms revealed that the isolate represents a novel species of the genus Knoellia, for which the name Knoellia flava sp. nov. is proposed; the type strain is TL1(T) ( = CGMCC 1.10749(T) = KCTC 19810(T)).
一株革兰氏阳性、需氧、不产芽孢的放线菌菌株，被命名为 TL1(T) 菌株，从中国武汉的猪粪中分离得到。细胞壁肽聚糖含有 meso-二氨基庚二酸。主要极性脂为磷脂酰乙醇胺、磷脂酰肌醇和双磷脂酰甘油。主要的menaquinone 为 MK-8(H(4))。主要脂肪酸（>10%）为 iso-C(16 : 0)、iso-C(15 : 0) 和 C(17 : 1)ω8c。基因组 DNA G+C 含量为 70.9 mol%。基于 16S rRNA 基因序列的系统发育分析表明，TL1(T) 菌株与中华游动放线菌（98.5%）、地下游动放线菌（98.2%）和 Aerolata 游动放线菌（96.9%）的模式株最为密切相关。TL1(T) 菌株与中华游动放线菌和地下游动放线菌的 DNA-DNA 同源性值分别为 27.3%和 34.0%。TL1(T) 菌株与相关生物的表型、化学分类和系统发育特征的比较表明，该分离株代表了游动放线菌属的一个新种，建议命名为黄色游动放线菌（Knoellia flava）sp. nov.；模式菌株为 TL1(T)（ = CGMCC 1.10749(T) = KCTC 19810(T)）。
