Institute of Microbiology, University of Innsbruck, Technikerstrasse 25, A-6020 Innsbruck, Austria.
DSMZ - Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH, Inhoffenstrasse 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):445-450. doi: 10.1099/ijs.0.031047-0. Epub 2011 Apr 1.
A gram-positive, non-motile, rod-shaped, psychrophilic actinomycete, designated strain Cr7-14(T), was isolated from alpine glacier cryoconite. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Cr7-14(T) was related to members of the genus Nocardioides and shared highest 16S rRNA gene sequence similarities with the type strains of Nocardioides furvisabuli (98.6 %), Nocardioides ganghwensis (98.2 %), Nocardioides oleivorans (98.1 %) and Nocardioides exalbidus (97.6 %). The predominant cellular fatty acids of strain Cr7-14(T) were C(17 : 1)ω8c (39.5 %) and iso-C(16 : 0) (32.4 %). The major menaquinone was MK-8(H(4)). The diagnostic diamino acid in the cell-wall peptidoglycan was ll-2,6-diaminopimelic acid. The predominant cell-wall sugars were galactose and rhamnose. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, four unknown glycolipids and two unknown polar lipids. The genomic DNA G+C content was 71.9 mol%. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness data, a novel species, Nocardioides alpinus sp. nov., is proposed, with Cr7-14(T) ( = DSM 23325(T) = LMG 26053(T) = CGMCC 1.10697(T)) as the type strain.
一株革兰氏阳性、非运动性、杆状、嗜冷放线菌，被命名为 Cr7-14(T) 菌株，从高山冰川冰核中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 Cr7-14(T) 与诺卡氏菌属的成员密切相关，与 Nocardioides furvisabuli（98.6%）、Nocardioides ganghwensis（98.2%）、Nocardioides oleivorans（98.1%）和 Nocardioides exalbidus（97.6%）的模式菌株的 16S rRNA 基因序列相似度最高。菌株 Cr7-14(T) 的主要细胞脂肪酸为 C(17 : 1)ω8c（39.5%）和 iso-C(16 : 0)（32.4%）。主要的menaquinone 为 MK-8(H(4))。细胞壁肽聚糖中的二氨基氨基酸是 ll-2,6-二氨基庚二酸。细胞壁糖主要为半乳糖和鼠李糖。极性脂谱含有双磷脂酰甘油、磷脂酰甘油、磷脂酰胆碱、磷脂酰肌醇、四种未知糖脂和两种未知极性脂。基因组 DNA G+C 含量为 71.9 mol%。基于表型特征、系统发育分析和 DNA-DNA 同源性数据，提出了一个新的物种，高山诺卡氏菌 Nocardioides alpinus sp. nov.，以 Cr7-14(T)（ = DSM 23325(T) = LMG 26053(T) = CGMCC 1.10697(T)）作为模式菌株。
