CERIC, Clinique Ambroise Paré 27, Neuilly, France.
Minerva Anestesiol. 2011 Mar;77(3):283-91.
The aim of the present study was to investigate the hormonal status and hemodynamic performance of a sheep model of congestive heart failure at rest and during maximum exercise.
The current investigation was a prospective, experimental study and was carried out in an experimental laboratory. Animals were randomly assigned to a congestive heart failure or control group. In the congestive heart failure group, sequential intracoronary embolization with 90-micron polystyrene microspheres was performed until the left ventricle ejection fraction fell below 35%. All animals were chronically instrumented to continuously monitor the pulmonary artery flow, right ventricle pressure, and left ventricle pressure. Blood samples were collected for hormone assays (atrial natriuretic factor, endothelin, renin plasmatic activity, noradrenaline, and adrenaline), and hemodynamic variables, including expired gases, were measured at baseline and during exercise.
Six animals in each group were studied. Compared to the controls, all of the studied plasma hormones were elevated in the model, and the most discriminative measurement was the atrial natriuretic factor (175±57 vs. 52±5 pg/mL, P<0.01). Exercise performance decreased by 30% to 40% in congestive heart failure animals compared to that of the controls (peak exercise VO2, 30±7 vs. 51±5 mL/min, P<0.01; anaerobic threshold, 21±7 vs. 37±2 mL/min, P<0.01; cardiac output, 201±33 vs. 350±45 mL/min.kg, P<0.01.
Intracoronary embolization in sheep produces a stable and reproducible model that acceptably simulates congestive heart failure in humans. This model is suitable for the long-term assessment of therapeutic strategies and was designed to improve exercise capabilities.
本研究旨在探讨充血性心力衰竭绵羊模型在休息和最大运动时的激素状态和血液动力学表现。
本研究为前瞻性实验研究，在实验室内进行。动物随机分为充血性心力衰竭组或对照组。在充血性心力衰竭组，通过序贯冠状动脉内栓塞 90 微米聚苯乙烯微球，直至左心室射血分数降至 35%以下。所有动物均被慢性植入仪器，以连续监测肺动脉流量、右心室压力和左心室压力。采集血液样本进行激素检测（心房利钠肽、内皮素、肾素血浆活性、去甲肾上腺素和肾上腺素），并在基线和运动期间测量血液动力学变量，包括呼出气体。
每组 6 只动物进行研究。与对照组相比，模型中所有研究的血浆激素均升高，最具鉴别力的测量指标是心房利钠肽（175±57 对 52±5 pg/mL，P<0.01）。与对照组相比，充血性心力衰竭动物的运动表现下降 30%至 40%（峰值运动 VO2，30±7 对 51±5 mL/min，P<0.01；无氧阈值，21±7 对 37±2 mL/min，P<0.01；心输出量，201±33 对 350±45 mL/min.kg，P<0.01）。
绵羊冠状动脉内栓塞可产生稳定且可重复的模型，可较好地模拟人类充血性心力衰竭。该模型适用于长期评估治疗策略，并旨在提高运动能力。
