Filippova T O, Britva I E, Golovenko N Ia, Popkov Iu A
Nauchnye Doki Vyss Shkoly Biol Nauki. 1990(5):36-41.
The effect of macroheterocyclic compounds possessing immunostimulating properties in vivo on the reactions of the immune splenocytes in vitro has been studied. It has been shown that the cell activity is determined by the medium ionic composition. In the medium with the optimal content of the bivalent cations the azacrown ethers decrease the intensity of the immune splenocytes reactions depending on the dose. In the media of the modified ionic composition the studied compounds in concentration 10 microM activate all the reactions of the spleen cells, in concentration 100 microM they suppress or do not change them.
研究了体内具有免疫刺激特性的大环杂环化合物对体外免疫脾细胞反应的影响。结果表明，细胞活性由培养基离子组成决定。在二价阳离子含量最佳的培养基中，氮杂冠醚会根据剂量降低免疫脾细胞反应的强度。在离子组成改变的培养基中，所研究的化合物在浓度为10微摩尔时会激活脾细胞的所有反应，在浓度为100微摩尔时会抑制这些反应或使其不变。
