利用近红外碳纳米管拉曼标签对癌细胞和肿瘤组织进行多色分子成像
Multiplexed Five-Color Molecular Imaging of Cancer Cells and Tumor Tissues with Carbon Nanotube Raman Tags in the Near-Infrared.
作者信息
Liu Zhuang, Tabakman Scott, Sherlock Sarah, Li Xiaolin, Chen Zhuo, Jiang Kaili, Fan Shoushan, Dai Hongjie
机构信息
Functional Nano & Soft Materials Laboratory (FUNSOM), Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Soochow University, Suzhou 215123, China.
出版信息
Nano Res. 2010 Jan 1;3(3):222-233. doi: 10.1007/s12274-010-1025-1.
Abstract
Single-walled carbon nanotubes (SWNTs) with five different C13/C12 isotope compositions and well-separated Raman peaks have been synthesized and conjugated to five targeting ligands in order to impart molecular specificity. Multiplexed Raman imaging of live cells has been carried out by highly specific staining of cells with a five-color mixture of SWNTs. Ex vivo multiplexed Raman imaging of tumor samples uncovers a surprising up-regulation of epidermal growth factor receptor (EGFR) on LS174T colon cancer cells from cell culture to in vivo tumor growth. This is the first time five-color multiplexed molecular imaging has been performed in the near-infrared (NIR) region under a single laser excitation. Near zero interfering background of imaging is achieved due to the sharp Raman peaks unique to nanotubes over the low, smooth autofluorescence background of biological species.
摘要
已合成出具有五种不同碳-13/碳-12同位素组成且拉曼峰分离良好的单壁碳纳米管（SWNTs），并将其与五种靶向配体偶联，以赋予分子特异性。通过用SWNTs的五色混合物对细胞进行高度特异性染色，对活细胞进行了多重拉曼成像。肿瘤样本的离体多重拉曼成像揭示了从细胞培养到体内肿瘤生长过程中，LS174T结肠癌细胞上表皮生长因子受体（EGFR）出现了惊人的上调。这是首次在单个激光激发下，在近红外（NIR）区域进行五色多重分子成像。由于纳米管独特的尖锐拉曼峰高于生物物种低而平滑的自发荧光背景，实现了近乎零干扰的成像背景。
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