Vinther O
Acta Pathol Microbiol Scand B. 1976 Aug;84(4):217-24. doi: 10.1111/j.1699-0463.1976.tb01928.x.
Measurements of urease activity of various cell fractions of U. urealyticum showed that this activity was confined to the soluble fraction of the cytoplasm. It was attempted to devise a method for electron microscopic detection of the sites of urease activity based on precipitation of electron dense MnO2 at the alkaline pH created by hydrolysis of urea. The results obtained by this method supported the previous results indicating a cytoplasmatic localization of the urease activity in the cells. Helical ribosome patterns were observed when glutaraldehyde fixed cells were treated with the cytochemical test solutions.
对解脲脲原体各种细胞组分的脲酶活性进行测量，结果表明该活性局限于细胞质的可溶部分。曾尝试设计一种基于在尿素水解产生的碱性pH条件下使电子致密的MnO₂沉淀，来对脲酶活性位点进行电子显微镜检测的方法。通过该方法获得的结果支持了先前表明脲酶活性定位于细胞细胞质中的结果。在用细胞化学测试溶液处理戊二醛固定的细胞时，观察到了螺旋状核糖体模式。
