CAPIM, Bio 21 Institute, University of Melbourne, Parkville, VIC 3052, Australia.
Bull Environ Contam Toxicol. 2011 May;86(5):461-4. doi: 10.1007/s00128-011-0251-x. Epub 2011 Mar 26.
In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hERα bioassay (r(2)=0.93), but not for the medERα bioassay (r(2)=0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurements were very good (r(2)>0.95). There was no correlation between bioassay response and ELISA ES measurements for estuarine samples.
在这项研究中，我们将两种基于酵母的重组受体-报告基因生物测定法与商业 ELISA 试剂盒进行了比较，以测量总雌激素。对于 WWTP 废水，ELISA 测量的总雌激素浓度与 hERα 生物测定法的雌激素活性（r(2)=0.93）非常相关，但与 medERα 生物测定法（r(2)=0.50）不相关。对于淡水样品，生物测定法反应与 ELISA ES 测量之间的相关性非常好（r(2)>0.95）。对于河口样本，生物测定法反应与 ELISA ES 测量之间没有相关性。
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