Bioorganic & Biophysical Chemistry Laboratory, Section for Biomaterials and Medicinal Chemistry, School of Natural Sciences, Linnaeus University, 39182 Kalmar, Sweden.
Anal Bioanal Chem. 2011 May;400(5):1397-404. doi: 10.1007/s00216-011-4907-5. Epub 2011 Mar 27.
A series of streptavidin-mimicking molecularly imprinted polymers has been developed and evaluated for their biotin binding characteristics. A combination of molecular dynamics and NMR spectroscopy was used to examine potential polymer systems, in particular with the functional monomers methacrylic acid and 2-acrylamidopyridine. The synthesis of copolymers of ethylene dimethacrylate and one or both of these functional monomers was performed. A combination of radioligand binding studies and surface area analyses demonstrated the presence of selectivity in polymers prepared using methacrylic acid as the functional monomer. This was predicted by the molecular dynamics studies showing the power of this methodology as a prognostic tool for predicting the behavior of molecularly imprinted polymers.
已经开发并评估了一系列模拟链霉亲和素的分子印迹聚合物，以研究它们与生物素的结合特性。本文采用分子动力学和 NMR 光谱相结合的方法来研究潜在的聚合物体系，特别是功能单体甲基丙烯酸和 2-丙烯酰胺吡啶。合成了二乙烯基苯和这两种功能单体之一或两者的共聚物。放射性配体结合研究和表面积分析的结合表明，使用甲基丙烯酸作为功能单体制备的聚合物具有选择性。这是通过分子动力学研究预测的，该研究表明该方法作为预测分子印迹聚合物行为的预测工具具有强大的功能。
