Kendall A, Hoffman-Goetz L, Houston M, MacNeil B, Arumugam Y
Department of Kinesiology, University of Waterloo, Ontario, Canada.
J Appl Physiol (1985). 1990 Jul;69(1):251-60. doi: 10.1152/jappl.1990.69.1.251.
This study examined the effect of exercise intensity and duration on the percent blood lymphocytes in men of low [LF; maximal O2 uptake (VO2max) less than 50 ml.kg-1.min-1 and sedentary], moderate (MF; VO2max = 50-60 ml.kg-1.min-1 and recreationally active), and high (HF; VO2max greater than 60 ml.kg-1.min-1 and recent training history) fitness. Thirty healthy adult men (aged 20-31 yr) participated in four randomly ordered cycle ergometer rides: ride 1 (65% VO2max, 30 min), ride 2 (30% VO2max, 60 min), ride 3 (75% VO2max, 60 min), and ride 4 (65% VO2max, 120 min). Blood samples were drawn at various times before and after the exercise sessions. Lymphocyte subsets were determined by flow cytometry using monoclonal antibodies for total T (CD3+), T-helper (CD4+), and T-suppressor (CD8+) lymphocytes and for a subset of cells expressing a natural killer (NK) cell antigen (Leu7+). Plasma catecholamines were assayed to determine exercise stress. There were sharp reductions (P less than 0.01) in the percentage of pan-T and T-helper lymphocytes immediately after exercise across all fitness levels; the magnitude of this reduction was greatest after the highest intensity (ride 3) or longest duration (ride 4) work. In contrast, the absolute number of T and T-helper cells tended to increase after exercise and significantly so in the HF subjects (P less than 0.005). There was no significant effect of exercise or subject fitness category on the percentage of T-suppressor lymphocytes, although the absolute numbers of this subset increased significantly after exercise in LF subjects. Marked increases (P less than 0.01) in the percentage of NK cells occurred immediately after exercise at all intensities and durations tested; numerical increases in total NK cells were significant in all fitness groups after the highest intensity work (ride 3; P less than 0.005). Irrespective of whether the changes were expressed as percentage or total numbers, recovery to base line occurred at 30 min after exercise. The results suggest that the exercise effect on blood lymphocyte subset percentages in men is transient and occurs across all fitness levels. Concomitant changes in plasma catecholamine concentrations are only weakly associated with these lymphocyte subset percentage responses to exercise. Furthermore, this study shows that the exercise-induced changes in lymphocyte percentages do not consistently reflect changes in the absolute numbers of cells.
本研究调查了运动强度和持续时间对低（LF；最大摄氧量（VO2max）小于50 ml·kg-1·min-1且久坐不动）、中（MF；VO2max = 50 - 60 ml·kg-1·min-1且有休闲运动习惯）、高（HF；VO2max大于60 ml·kg-1·min-1且有近期训练经历）健康水平男性血液淋巴细胞百分比的影响。30名健康成年男性（年龄20 - 31岁）参与了四次随机安排的蹬自行车运动：骑行1（65%VO2max，30分钟）、骑行2（30%VO2max，60分钟）、骑行3（75%VO2max，60分钟）和骑行4（65%VO2max，120分钟）。在运动前后的不同时间采集血样。使用针对总T（CD3+）、辅助性T（CD4+）和抑制性T（CD8+）淋巴细胞以及表达自然杀伤（NK）细胞抗原的细胞亚群（Leu7+）的单克隆抗体，通过流式细胞术测定淋巴细胞亚群。测定血浆儿茶酚胺以确定运动应激。在所有健康水平下，运动后即刻全T淋巴细胞和辅助性T淋巴细胞的百分比均急剧下降（P < 0.01）；这种下降幅度在最高强度（骑行3）或最长持续时间（骑行4）运动后最大。相比之下，运动后T淋巴细胞和辅助性T淋巴细胞的绝对数量趋于增加，在HF组中增加显著（P < 0.005）。运动或受试者健康水平类别对抑制性T淋巴细胞百分比没有显著影响，尽管LF组中该亚群的绝对数量在运动后显著增加。在所有测试的强度和持续时间下，运动后即刻NK细胞百分比均显著增加（P < 0.01）；在最高强度运动（骑行3）后，所有健康组中NK细胞总数的增加均显著（P < 0.005）。无论变化是以百分比还是总数表示，运动后30分钟恢复至基线水平。结果表明，运动对男性血液淋巴细胞亚群百分比的影响是短暂的，且在所有健康水平下均会出现。血浆儿茶酚胺浓度的相应变化与这些淋巴细胞亚群百分比对运动的反应仅存在微弱关联。此外，本研究表明，运动诱导的淋巴细胞百分比变化并不始终反映细胞绝对数量的变化。
