Shinkai S, Shore S, Shek P N, Shephard R J
Department of Hygiene, Ehime University School of Medicine, Japan.
Int J Sports Med. 1992 Aug;13(6):452-61. doi: 10.1055/s-2007-1021297.
Twenty-one young male subjects exercised on a cycle ergometer for 60 min at 60% of VO2max. Blood samples collected every 30 min throughout exercise and continuing to 120 min recovery served for the immunological tests. Exercise induced biphasic changes in the various leucocyte subsets. There was a granulocytosis, lymphocytosis and monocytosis during exercise, and a further granulocytosis and a slight monocytosis, but a lymphocytopenia during recovery. All lymphocyte subsets (CD3+, CD19+, CD4+, CD8+, and CD16+ cells) increased in number during exercise, were decreased 30 min after exercise, and had not returned to baseline levels by 120 min of recovery. The apparent lymphocyte responsiveness to the mitogens phytohaemagglutinin (PHA) and pokeweed mitogen (PWM) declined significantly during exercise, returning to normal by 120 min of recovery. The natural killer (NK) activity rose markedly during exercise, but decreased to almost half the pre-exercise level at 30 and 60 min of recovery, returning to baseline levels after 120 min of recovery. Functional capability correlated well with the percentage of each major responder subset in the assay, suggesting that the in vitro lymphocyte PHA- and PWM-responsiveness and the NK activity did not change significantly on a per cell basis. The analysis of lymphocyte marker antigen density revealed that the CD3+, CD4+, CD8+ and CD19+ lymphocytes mobilized into the circulation during exercise did not express the respective CD3, CD4, CD8 and CD19 molecules as strongly as did the subsets circulating at rest, whereas the expression of the CD16 antigen on CD16+ lymphocytes remained unchanged.
21名年轻男性受试者在功率自行车上以最大摄氧量（VO2max）的60%进行60分钟运动。在运动过程中每30分钟采集一次血样，并持续至恢复120分钟，用于免疫测试。运动引起了各种白细胞亚群的双相变化。运动期间出现粒细胞增多、淋巴细胞增多和单核细胞增多，恢复期间则出现进一步的粒细胞增多和轻微的单核细胞增多，但淋巴细胞减少。所有淋巴细胞亚群（CD3 +、CD19 +、CD4 +、CD8 +和CD16 +细胞）在运动期间数量增加，运动后30分钟减少，并且在恢复120分钟时仍未恢复到基线水平。运动期间，淋巴细胞对丝裂原植物血凝素（PHA）和商陆有丝分裂原（PWM）的明显反应性显著下降，在恢复120分钟时恢复正常。自然杀伤（NK）活性在运动期间显著升高，但在恢复30分钟和60分钟时降至运动前水平的近一半，在恢复120分钟后恢复到基线水平。功能能力与检测中每个主要反应亚群的百分比密切相关，这表明体外淋巴细胞PHA和PWM反应性以及NK活性在单个细胞基础上没有显著变化。淋巴细胞标志物抗原密度分析显示，运动期间动员到循环中的CD3 +、CD4 +、CD8 +和CD19 +淋巴细胞表达各自的CD3、CD4、CD8和CD19分子的强度不如静息时循环的亚群，而CD16 +淋巴细胞上CD16抗原的表达保持不变。
