Hong Suzi, Johnson Todd A, Farag Noha H, Guy Harold J, Matthews Scott C, Ziegler Michael G, Mills Paul J
Department of Psychiatry, University of California, 9500 Gilman Dr., La Jolla, CA 92093-0804, USA.
J Appl Physiol (1985). 2005 Mar;98(3):1057-63. doi: 10.1152/japplphysiol.00233.2004. Epub 2004 Oct 22.
The effects of physical fitness on leukocyte demargination and cellular adhesion molecule (CAM) responses to moderate exercise were examined. We assessed leukocyte subsets and CAM expression before, immediately after, and 10 min after a 20-min treadmill exercise at 65-70% peak oxygen consumption in fit vs. nonfit individuals. Physical fitness was determined by peak oxygen consumption during a treadmill test. Catecholamine levels were determined by radioenzymatic assay, and enumeration of cells and detection of CAM expression were assessed by flow cytometry. As expected, exercise led to significant increases in numbers of leukocyte subsets, regardless of fitness level (P < 0.01). Values returned to near resting levels 10 min after exercise. More importantly, physically fit individuals showed attenuated responses to the moderate-exercise challenge in numbers of CD3(+), CD4(+), CD8(+), memory (CD45RO(+)) CD4, and naive (CD45RA(+)62L(+)) CD4 and CD8 lymphocytes. Postexercise human leukocyte antigen-DR absent memory CD4(+) cell numbers were also lower in fit subjects. Increases in CD62L-expressing CD4(+) and CD8(+) lymphocytes and CD11a- expressing lymphocytes after exercise were also attenuated in fit individuals compared with nonfit individuals (P < 0.05). Catecholamine levels increased to a similar extent (P < 0.01) in both fitness groups. The findings suggest that physical fitness attenuates demargination of selected lymphocyte subsets in response to moderate exercise. Although the differences in plasma catecholamine responses were not significant between the groups, a possible mediating role of the sympathetic system remains to be further investigated. Being physically fit may offset exaggerated immune cell responses to stress.
研究了身体素质对白细胞边缘移动以及细胞粘附分子（CAM）对适度运动反应的影响。我们评估了健康个体与非健康个体在以65 - 70%峰值耗氧量进行20分钟跑步机运动前、运动后即刻以及运动后10分钟时的白细胞亚群和CAM表达情况。身体素质通过跑步机测试中的峰值耗氧量来确定。儿茶酚胺水平通过放射酶法测定，细胞计数和CAM表达检测通过流式细胞术进行评估。正如预期的那样，无论身体素质水平如何，运动都会导致白细胞亚群数量显著增加（P < 0.01）。运动后10分钟，数值恢复到接近静息水平。更重要的是，健康个体在CD3(+)、CD4(+)、CD8(+)、记忆性（CD45RO(+)）CD4以及初始（CD45RA(+)62L(+)）CD4和CD8淋巴细胞数量对适度运动挑战的反应中表现出减弱。运动后健康受试者中缺乏人类白细胞抗原 - DR的记忆性CD4(+)细胞数量也较低。与非健康个体相比，健康个体运动后表达CD62L的CD4(+)和CD8(+)淋巴细胞以及表达CD11a的淋巴细胞的增加也有所减弱（P < 0.05）。两个身体素质组的儿茶酚胺水平升高程度相似（P < 0.01）。这些发现表明，身体素质会减弱特定淋巴细胞亚群对适度运动的边缘移动反应。尽管两组之间血浆儿茶酚胺反应的差异不显著，但交感神经系统可能的介导作用仍有待进一步研究。身体健康可能会抵消免疫细胞对应激的过度反应。
