SOS修复对单链DNA的效果可能与对双链DNA的效果一样好，甚至更好。
SOS repair can be about as effective for single-stranded DNA as for double-stranded DNA and even more so.
作者信息
Tessman I
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
出版信息
J Bacteriol. 1990 Sep;172(9):5503-5. doi: 10.1128/jb.172.9.5503-5505.1990.
As ordinarily measured, the SOS repair of damaged DNA by Weigle reactivation appears to be more effective for double-stranded (ds) than for single-stranded (ss) DNA bacteriophages. A complicating feature, which is usually not considered, is the possibility of DNA-protein cross-linking of ssDNA to the viral capsid, which would conceivably be an extraneous source of nonreactivable lesions. This idea is supported in studies of phage S13 by the observation that photoreactivation more than doubles when naked ssDNA is substituted for encapsidated ssDNA as the UV target. The same effect was observed for Weigle reactivation; there was little, if any, difference in the reactivation of ssDNA and dsDNA when naked DNA was irradiated. Moreover, in a uvrA mutant, ssDNA actually had the advantage; Weigle reactivation was then more than twice as effective for ssDNA as for dsDNA. It is also shown that when a suitable measure of Weigle mutagenesis is used, there is no convincing evidence that dsDNA is mutagenized more effectively than ssDNA.
按照常规测量方法，通过韦格勒复活作用对受损DNA进行的SOS修复，对于双链（ds）DNA噬菌体而言似乎比对单链（ss）DNA噬菌体更有效。一个通常未被考虑的复杂因素是，单链DNA与病毒衣壳发生DNA - 蛋白质交联的可能性，这可以想象是不可复活损伤的一个外来来源。在对噬菌体S13的研究中，当用裸露的单链DNA替代被包裹的单链DNA作为紫外线照射目标时，光复活作用增加了一倍多，这一观察结果支持了这一观点。韦格勒复活作用也观察到了同样的效果；当照射裸露的DNA时，单链DNA和双链DNA的复活作用几乎没有差异（如果有差异也很小）。此外，在uvrA突变体中，单链DNA实际上具有优势；此时韦格勒复活作用对单链DNA的效果比对双链DNA的效果高出两倍多。研究还表明，当使用合适的韦格勒诱变测量方法时，没有令人信服的证据表明双链DNA比单链DNA更容易发生诱变。
Zotero 插件