Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Antiviral Res. 2011 Jun;90(3):134-42. doi: 10.1016/j.antiviral.2011.03.185. Epub 2011 Apr 5.
It is well documented that adjuvants improve the immune response generated by traditional viral vaccines; however, less is known about their effects on the immune response elicited by DNA vaccines. In this study, we have investigated the use of adjuvants, and have analyzed the humoral and cellular specific immune responses elicited by DNA vaccines based on the BoHV-1 glycoprotein D (secreted version) in pCIneo vector with and without Montanide ISA25 (O/W), ISA206 VG (SEPPIC) and Cliptox™ (natural microparticles of clinoptilolite). The comparison of the immune response induced in mice by pCIgD formulated with or without adjuvants showed that the immunomodulators affect the total specific humoral and cellular response. The isotypes induced by these adjuvants were of the type Th1/Th2. A significant increase in the mac-3+ and F4/80+ populations of the groups receiving pCIneo with ISA25, ISA206; and an increase in CD4+ populations of the group receiving pCIneo ISA25, in comparison with the pCIneo group was observed. On the other hand, mice vaccinated with pCIgD/ISA25, pCIgD/ISA206, or pCIgD/Cliptox developed a significantly higher specific cytotoxic activity against BoHV-1 than the pCIgD and pCIneo groups. In this report we propose the use of ISA25, ISA206 or Cliptox as adjuvants in a DNA vaccine since they are able to induce not only a specific humoral immune response but also a specific cellular immune response.
有大量文献记载佐剂可增强传统病毒疫苗产生的免疫应答；然而，关于它们对 DNA 疫苗引发的免疫应答的影响知之甚少。在这项研究中，我们研究了佐剂的应用，并分析了基于 BoHV-1 糖蛋白 D（分泌型）的 DNA 疫苗在 pCIneo 载体中的体液和细胞特异性免疫应答，佐剂包括 Montanide ISA25（O/W）、ISA206 VG（SEPPIC）和 Cliptox™（天然沸石微球）。比较了用或不用佐剂配制的 pCIgD 在小鼠中引发的免疫应答，结果表明免疫调节剂会影响总特异性体液和细胞应答。这些佐剂诱导的同种型为 Th1/Th2 型。与 pCIneo 组相比，接受 pCIneo 与 ISA25、ISA206 联合免疫的小鼠中 mac-3+和 F4/80+群体增加，而接受 pCIneo ISA25 免疫的小鼠中 CD4+群体增加。另一方面，与 pCIgD 和 pCIneo 组相比，接种 pCIgD/ISA25、pCIgD/ISA206 或 pCIgD/Cliptox 的小鼠针对 BoHV-1 的特异性细胞毒性活性显著升高。在本报告中，我们提议使用 ISA25、ISA206 或 Cliptox 作为 DNA 疫苗佐剂，因为它们不仅能诱导特异性体液免疫应答，还能诱导特异性细胞免疫应答。
