Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Apr 15;879(13-14):938-44. doi: 10.1016/j.jchromb.2011.03.003. Epub 2011 Mar 10.
Plant hormones play crucial roles in plant growth and development. However, up to date, identification and quantification of acidic plant hormones with trace amount in complicated plant matrix is still a challenge. In current study, we developed a high sensitive assay for the determination of acidic plant hormones in rice by combining capillary electrophoresis and electrospray ionization-time of flight-mass spectrometry (CE-ESI-TOF-MS). To improve the detection sensitivity of acidic plant hormones, 3-bromoactonyltrimethylammonium bromide (BTA) was synthesized as a new mass probe, which can react efficiently with acidic plant hormones in acetonitrile containing triethylamine (TEA). The positively charged BTA-derivatives were separated by CE using amino-coated capillary, which provided a reversed electroosmotic flow (EOF) at low pH, as well as reduced the adsorption of BTA-derivatives on the inner wall of capillary. Using the CE-ESI-TOF-MS method developed in current study, 15 acidic plant hormones, including 10 gibberellins (GAs), were identified and quantified with good linearities from 1.3 to 850 ng/mL with linear coefficient R(2) values of >0.99. The limits of detection (LODs) were in the range of 0.34-4.59 ng/mL. Recoveries of compounds from spiked beverage samples ranged from 84.6 to 112.2%. And a good reproducibility was obtained by evaluating the intra and inter-day precisions with relative standard deviations (RSDs) less than 6.7 and 9.9%, respectively.
植物激素在植物生长和发育中起着至关重要的作用。然而，迄今为止，在复杂的植物基质中鉴定和定量痕量的酸性植物激素仍然是一个挑战。在当前的研究中，我们通过结合毛细管电泳和电喷雾电离飞行时间质谱（CE-ESI-TOF-MS），开发了一种用于测定水稻中酸性植物激素的高灵敏度方法。为了提高酸性植物激素的检测灵敏度，我们合成了 3-溴丙酰三甲基氯化铵（BTA）作为一种新的质量探针，它可以在含有三乙胺（TEA）的乙腈中与酸性植物激素有效反应。带正电荷的 BTA 衍生物通过涂有氨基的毛细管进行 CE 分离，在低 pH 值下提供了反向电渗流（EOF），并减少了 BTA 衍生物在毛细管内壁上的吸附。使用当前研究中开发的 CE-ESI-TOF-MS 方法，鉴定和定量了 15 种酸性植物激素，包括 10 种赤霉素（GAs），线性范围从 1.3 到 850ng/mL，线性系数 R（2）值大于 0.99。检出限（LODs）范围为 0.34-4.59ng/mL。从加标饮料样品中回收的化合物回收率在 84.6-112.2%之间。通过评估日内和日间精密度，相对标准偏差（RSDs）分别小于 6.7%和 9.9%，获得了良好的重现性。
