过量表达玉米基因可通过调控赤霉素的生物合成降低转基因水稻株高。
Overexpression of the maize gene in transgenic rice reduce plant height by regulating the biosynthesis of GA.
机构信息
National Engineering Laboratory of Crop Stress Resistance Breeding, School of Life Sciences, Anhui Agricultural University, Hefei, China.
School of Life Sciences, Anhui Agricultural University, Hefei, China.
出版信息
Plant Signal Behav. 2021 Nov 2;16(11):1967635. doi: 10.1080/15592324.2021.1967635. Epub 2021 Sep 9.
Abstract
WRKYs represent an important family of transcription factors that are widely involved in plant development, defense regulation and stress response. Transgenic rice that constitutively expressed had shorter plant height and showed less sensitivity to gibberellic acid (GA). Further investigation proved that transgenic rice accumulated lower levels of bioactive GAs than that in wild-type plants. Application of exogenous GA fully rescued the semi-dwarf phenotype of transgenic plants. Transcriptome and qRT-PCR analyses indicated that the expression of , encoding the repressor of GA biosynthesis, was markedly increased. Electrophoretic mobility shift assay and dual-luciferase reporter assay indicated that ZmWRKY114 directly binds to a W-box motif in the promoter. Taken together, these results confirm that is a GA-responsive gene and is participated in the regulation of plant height in rice.
摘要
WRKY 蛋白是一类重要的转录因子家族，广泛参与植物的发育、防御调控和应激反应。组成型表达的转基因水稻植株较矮，对赤霉素（GA）的敏感性降低。进一步的研究证明，转基因水稻中生物活性 GA 的积累水平低于野生型植物。外源 GA 的应用完全挽救了 转基因植物的半矮化表型。转录组和 qRT-PCR 分析表明，编码 GA 生物合成抑制剂的 的表达显著增加。电泳迁移率变动分析和双荧光素酶报告基因分析表明，ZmWRKY114 直接结合到 启动子中的 W-box 基序上。综上所述，这些结果证实 是一个对 GA 响应的基因，并参与了水稻株高的调控。
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