Schlegelová J, Sedivá I, Opletal A, Rysánek D
Výzkumný ústav veterinárniho lékarství.
Cesk Epidemiol Mikrobiol Imunol. 1990 Jun;39(3):155-60.
The author used the haemagglutination method to estimate A protein in 153 strains of Staphylococcus aureus from collections. Protein A was detected in 66% of coagulase positive strains deposited in the course of time in the collection in 1983-1988. All strains were kept in skimmed milk at -20 degrees C. For evaluation of the reaction a semiquantitative scale was proposed. The author observed a diminished capacity to form protein A with the time of storage of the strains. In strains deposited for a shorter time in the collection (only three years) the percentage of positive results rose to 92.5%. In coagulase negative strains protein A was not detected.
作者采用血凝法对收集的153株金黄色葡萄球菌中的A蛋白进行了评估。在1983年至1988年期间收集的凝固酶阳性菌株中,66%检测到了蛋白A。所有菌株均保存在-20℃的脱脂牛奶中。为评估反应,提出了一个半定量标准。作者观察到,随着菌株保存时间的延长,形成蛋白A的能力下降。在收集后保存时间较短(仅三年)的菌株中,阳性结果的百分比升至92.5%。在凝固酶阴性菌株中未检测到蛋白A。