Hayward A R, Clarke J, Cosyns M
Department of Pediatrics, University of Colorado School of Medicine, Denver 80262.
Clin Exp Immunol. 1990 Sep;81(3):475-8. doi: 10.1111/j.1365-2249.1990.tb05358.x.
Blood mononuclear cells (MNC) expressing the T cell receptor V beta gene families were identified with the monoclonal antibodies 1C1 (V beta 5) or Mx6 (V beta 8). These cells were typed for expression of the T200 common leucocyte antigen with the CD45RA antibody defining the 220-kD variant (which characterizes naive cells) and the UCHL1 antibody which defines the 180-kD variant (characterizing memory cells). Of adult MNC, 3 +/- 0.44% stained for V beta 5 and 4.12 +/- 0.26% stained for V beta 8; similar results were obtained in a group of infants aged less than 3 months, who were exposed to antigen stimulus in the form of infection and/or blood transfusion. While the total percentages of V beta 5 and V beta 8 cells in the blood of adults were not correlated, the proportions of these cells expressing CD45RO was positively correlated. A similar trend which did not reach statistical significance was present in a group of infants. A minority of the infants studied showed transient deviations from the mean established for adults. The results suggest that naive and memory T cell populations have similar frequencies of V beta 5 and V beta 8 expressing cells, and that age does not affect the frequency of usage of either of these V beta families.
用单克隆抗体1C1（Vβ5）或Mx6（Vβ8）鉴定表达T细胞受体Vβ基因家族的血液单核细胞（MNC）。用CD45RA抗体（定义220-kD变体，该变体是幼稚细胞的特征）和UCHL1抗体（定义180-kD变体，该变体是记忆细胞的特征）对这些细胞进行T200共同白细胞抗原表达分型。在成人MNC中，3±0.44%的细胞Vβ5染色阳性，4.12±0.26%的细胞Vβ8染色阳性；在一组小于3个月的婴儿中也得到了类似结果，这些婴儿受到感染和/或输血形式的抗原刺激。虽然成人血液中Vβ5和Vβ8细胞的总百分比不相关，但这些表达CD45RO的细胞比例呈正相关。一组婴儿中也存在类似趋势，但未达到统计学意义。少数研究的婴儿显示出与成人确定的平均值有短暂偏差。结果表明，幼稚和记忆T细胞群体中表达Vβ5和Vβ8的细胞频率相似，并且年龄不影响这两个Vβ家族中任何一个的使用频率。
