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表达特定T细胞抗原受体家族的人T淋巴细胞可特异性杀伤产生抗受体单克隆抗体的杂交瘤细胞。

Human T lymphocytes expressing a defined T-cell antigen receptor family specifically kill the hybridoma that makes the anti-receptor monoclonal antibody.

作者信息

Boylston A W, May S J

出版信息

Immunology. 1986 Nov;59(3):383-7.

PMID:3491783
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1453196/
Abstract

Human T lymphocytes expressing a family of T-cell antigen receptors defined by a particular monoclonal antibody can be induced to grow with the Mab and recombinant IL-2. The resulting population consists largely of cells that have the phenotype T3+, T8+, T4-, and that react with the inducing anti-TcR Mab. The ability of such T cells to kill the hybridoma which produces the inducing Mab has been investigated. Specific killing is demonstrated which is inhibited by Mab to T3, TcR, and LFA-1. Further, lytic activity is rapidly lost when cultures are deprived of lymphokines. Cytolytic activity is restored by incubating cells in recombinant IL-2.

摘要

表达由一种特定单克隆抗体定义的T细胞抗原受体家族的人T淋巴细胞，可被诱导与该单克隆抗体和重组白细胞介素-2一起生长。产生的细胞群体主要由具有T3+、T8+、T4-表型且能与诱导性抗T细胞受体单克隆抗体发生反应的细胞组成。已经研究了这类T细胞杀死产生诱导性单克隆抗体的杂交瘤的能力。结果表明存在特异性杀伤作用，这种作用可被针对T3、T细胞受体和淋巴细胞功能相关抗原-1的单克隆抗体所抑制。此外，当培养物中缺乏淋巴因子时，溶解活性会迅速丧失。通过在重组白细胞介素-2中培养细胞，细胞溶解活性得以恢复。

相似文献

1
Human T lymphocytes expressing a defined T-cell antigen receptor family specifically kill the hybridoma that makes the anti-receptor monoclonal antibody.表达特定T细胞抗原受体家族的人T淋巴细胞可特异性杀伤产生抗受体单克隆抗体的杂交瘤细胞。
Immunology. 1986 Nov;59(3):383-7.
2
Anti-T3 antibody both activates and inhibits the cytotoxic activity of human T cell clones.抗T3抗体既能激活又能抑制人T细胞克隆的细胞毒性活性。
Behring Inst Mitt. 1985 Aug(77):30-8.
3
A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among anti-Ia cytolytic T cell clones.一种大鼠抗小鼠T4单克隆抗体（H129.19）可抑制Ia反应性T细胞克隆的增殖，并在抗Ia细胞毒性T细胞克隆中区分出两个表型不同的亚群（T4+、Lyt-2,3-和T4-、Lyt-2,3+）。
J Immunol. 1984 Jun;132(6):2775-82.
4
Monoclonal cytotoxic T lymphocyte hybridomas capable of specific killing activity, antigenic responsiveness, and inducible interleukin secretion.具有特异性杀伤活性、抗原反应性和诱导性白细胞介素分泌能力的单克隆细胞毒性T淋巴细胞杂交瘤。
J Immunol. 1983 Jul;131(1):50-6.
5
Properties of a panel of monoclonal antibodies which react with the human T cell antigen receptor on the leukemic line HPB-ALL and a subset of normal peripheral blood T lymphocytes.一组单克隆抗体的特性，这些抗体可与白血病细胞系HPB-ALL及一部分正常外周血T淋巴细胞上的人类T细胞抗原受体发生反应。
J Immunol. 1986 Jul 15;137(2):741-4.
6
Use of I region-restricted, antigen-specific T cell hybridomas to produce idiotypically specific anti-receptor antibodies.使用I区限制的、抗原特异性T细胞杂交瘤来产生独特型特异性抗受体抗体。
J Immunol. 1983 Mar;130(3):1033-7.
7
Monoclonal anti-CD44 antibody acts in synergy with anti-CD2 but inhibits anti-CD3 or T cell receptor-mediated signaling in murine T cell hybridomas.单克隆抗CD44抗体与抗CD2协同作用，但在小鼠T细胞杂交瘤中抑制抗CD3或T细胞受体介导的信号传导。
Cell Immunol. 1993 Nov;152(1):186-99. doi: 10.1006/cimm.1993.1278.
8
Use of bispecific heteroconjugated antibodies (anti-T cell antigen receptor x anti-MHC class II) to study activation of T cells with a full length or truncated antigen receptor zeta-chain.使用双特异性异源缀合抗体（抗T细胞抗原受体x抗MHC II类）研究具有全长或截短抗原受体ζ链的T细胞激活。
J Immunol. 1993 Mar 15;150(6):2211-21.
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Characterization and expression of the human alpha beta T cell receptor by using a framework monoclonal antibody.利用一种框架单克隆抗体对人αβ T细胞受体进行表征与表达研究
J Immunol. 1987 Mar 1;138(5):1502-9.
10
Requirements for T cell activation by OKT3 monoclonal antibody: role of modulation of T3 molecules and interleukin 1.OKT3单克隆抗体激活T细胞的要求：T3分子调节和白细胞介素1的作用。
J Immunol. 1985 Sep;135(3):1714-8.

引用本文的文献

1
Lysis of CD3 hybridoma targets by cloned human CD4 lymphocytes.克隆的人CD4淋巴细胞对CD3杂交瘤靶细胞的裂解作用。
Immunology. 1988 May;64(1):87-92.
2
V beta 5 and V beta 8 memory T cells in adults and infancy: co-ordinated increase in response to early antigen stimulus.成人和婴儿体内的Vβ5和Vβ8记忆性T细胞：对早期抗原刺激反应的协同增加。
Clin Exp Immunol. 1990 Sep;81(3):475-8. doi: 10.1111/j.1365-2249.1990.tb05358.x.

本文引用的文献

1
Antigen-like effects of monoclonal antibodies directed at receptors on human T cell clones.针对人T细胞克隆受体的单克隆抗体的抗原样效应。
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2
Regulation of influenza virus-specific cytotoxic T cell responses by monoclonal antibody to a human T cell differentiation antigen.针对人类T细胞分化抗原的单克隆抗体对流感病毒特异性细胞毒性T细胞反应的调节
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The human T-cell receptor.人类T细胞受体。
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Attachment of an anti-receptor antibody to non-target cells renders them susceptible to lysis by a clone of cytotoxic T lymphocytes.抗受体抗体与非靶细胞的结合使它们易于被细胞毒性T淋巴细胞克隆裂解。
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6
Immune responses in vitro. I. Culture conditions for antibody synthesis.体外免疫反应。I. 抗体合成的培养条件。
Cell Immunol. 1972 Feb;3(2):264-76. doi: 10.1016/0008-8749(72)90165-7.
7
Activation through CD3 molecule leads to clonal expansion of all human peripheral blood T lymphocytes: functional analysis of clonally expanded cells.通过CD3分子激活可导致所有人外周血T淋巴细胞的克隆性扩增：对克隆性扩增细胞的功能分析。
J Immunol. 1985 Oct;135(4):2337-42.
8
Growth of normal human T lymphocytes induced by monoclonal antibody to the T cell antigen receptor.抗T细胞抗原受体单克隆抗体诱导正常人T淋巴细胞的生长
Eur J Immunol. 1985 Jul;15(7):738-42. doi: 10.1002/eji.1830150719.
9
On the mechanism of unidirectional killing in mixtures of two cytotoxic T lymphocytes. Unidirectional polarization of cytoplasmic organelles and the membrane-associated cytoskeleton in the effector cell.关于两种细胞毒性T淋巴细胞混合物中单向杀伤的机制。效应细胞中细胞质细胞器和膜相关细胞骨架的单向极化。
J Exp Med. 1986 Mar 1;163(3):489-98. doi: 10.1084/jem.163.3.489.
10
Induction of nonspecific cytotoxicity by monoclonal anti-T3 antibodies.单克隆抗-T3抗体诱导非特异性细胞毒性
J Immunol. 1985 Jun;134(6):3770-5.