Leprince C, Cohen-Kaminsky S, Berrih-Aknin S, Vernet-Der Garabedian B, Treton D, Galanaud P, Richard Y
INSERM U.131, Clamart, France.
J Immunol. 1990 Oct 1;145(7):2115-22.
Thymic cell populations from 12 patients displaying myasthenia gravis were submitted to a phenotypic and functional study. Immunofluorescence analysis of thymic sections revealed the presence in germinal centers of B lymphocytes expressing the B cell markers--CD19, CD21, IgD, or IgM. After T cell and macrophage depletion of thymic single cell suspensions, B cell-enriched populations were isolated. Enriched B cells expressed at variable levels activation markers such as CD71, 4F2, CD23, and B8.7, indicating that a marked proportion of them are activated. Moreover, addition of B cell growth factor 12kDa and to a lesser extent of rIL-2 induced a spontaneous proliferation of these B cell populations. These functional and phenotypic signs of activation may reveal the first steps of an autoimmune response against acetylcholine receptor as enriched B cell populations have the capacity to spontaneously secrete anti-acetylcholine receptor antibody.
对12例重症肌无力患者的胸腺细胞群体进行了表型和功能研究。胸腺切片的免疫荧光分析显示，生发中心存在表达B细胞标志物——CD19、CD21、IgD或IgM的B淋巴细胞。在对胸腺单细胞悬液进行T细胞和巨噬细胞清除后，分离出富含B细胞的群体。富集的B细胞以不同水平表达激活标志物，如CD71、4F2、CD23和B8.7，表明其中相当一部分被激活。此外，添加12kDa B细胞生长因子以及少量rIL-2可诱导这些B细胞群体自发增殖。这些激活的功能和表型特征可能揭示了针对乙酰胆碱受体的自身免疫反应的最初步骤，因为富集的B细胞群体有能力自发分泌抗乙酰胆碱受体抗体。
