Schlüter B, König W, Köller M, Erbs G, Müller F E
Lehrstuhl für Med. Mikrobiologie und Immunologie, Ruhr-Universität Bochum, F.R.G.
J Trauma. 1991 Feb;31(2):239-46.
We studied the in vitro expression and regulation of the CD23 and CD25 (Tac) surface antigens by peripheral blood lymphocytes (PBL) from severely burned patients (burn injuries ranging from 25% to 72% TBSA) in order to evaluate T- and B-lymphocyte activation processes after thermal trauma. The spontaneous and cytokine (IL-4, IL-2)-induced expression of CD23 which represents a B-cell activation marker was significantly reduced during the second to fifth week postburn when compared to healthy donors. In contrast, CD25, which is expressed on activated T cells, showed a marked increase both spontaneously, indicating an in vivo activation, and after stimulation with IL-2 or PHA. Concomitantly, T-cell proliferation induced by PHA or Con A was suppressed. However, the number of T and B cells remained unchanged. The data demonstrate the impairment of early events in the lymphocyte program in severely burned patients. The activation of B cells is downregulated, since they become refractory to external helper signals. In addition, T cells are highly activated but fail to proceed to proliferation in response to mitogenic stimuli.
我们研究了重度烧伤患者（烧伤面积占总体表面积的25%至72%）外周血淋巴细胞（PBL）对CD23和CD25（Tac）表面抗原的体外表达及调控情况，以评估热损伤后T淋巴细胞和B淋巴细胞的激活过程。与健康供体相比，代表B细胞激活标志物的CD23在烧伤后第二至五周的自发表达以及细胞因子（IL-4、IL-2）诱导的表达均显著降低。相比之下，在活化T细胞上表达的CD25，无论是自发表达（表明体内激活），还是在用IL-2或PHA刺激后，均显示出显著增加。同时，PHA或Con A诱导的T细胞增殖受到抑制。然而，T细胞和B细胞的数量保持不变。这些数据表明重度烧伤患者淋巴细胞程序中的早期事件受到损害。B细胞的激活被下调，因为它们对外源辅助信号变得不敏感。此外，T细胞被高度激活，但对有丝分裂刺激无反应而无法进行增殖。
