Laboratory of Food safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
J Sci Food Agric. 2011 Jul;91(9):1630-7. doi: 10.1002/jsfa.4359. Epub 2011 Mar 28.
Antifungal proteins (AFP) help plants to combat phytopathogenic fungi and thus protect plants from the devastating damage caused by fungal infections and prevent massive economic losses. To date, several proteins with antibacterial and/or antifungal properties have been isolated and characterized from different plant species and tissues; however, there are no reports concerning the antifungal peptide from foxtail millet seeds.
An antifungal peptide with a molecular mass of 26.9 kDa was isolated from dry seeds of the foxtail millet (Setaria italica (L.) Beauv.), using a procedure that involved four chromatographic steps. The antifungal peptide was adsorbed on CM-Sepharose, Affi-gel blue gel and Superdex 75. It was further purified by C(18) reverse-phase high-performance liquid chromatography and submitted for analysis of peptide mass fingerprint. The Mascot peptide mass fingerprint of the isolated protein hit no existing protein (score >60), and it was proved to be a novel antifungal peptide. It inhibited mycelial growth in Alternaria alternate with an IC(50) of 1.3 µmol L(-1) , and it also exhibited antifungal activity against Trichoderma viride, Botrytis cinerea and Fusarium oxysporum. Transmission electron microscopy of mold forms of Alternaria alternate after incubation with 20 µg mL(-1) of the antifungal protein for 48 h revealed marked ultrastructural changes in the fungus.
A novel antifungal peptide with high potency was isolated from foxtail millet seeds.
抗菌蛋白（AFP）有助于植物对抗植物病原菌真菌，从而保护植物免受真菌感染造成的破坏性损害，并防止巨大的经济损失。迄今为止，已经从不同的植物物种和组织中分离和鉴定出几种具有抗菌和/或抗真菌特性的蛋白质；然而，目前尚无关于来自谷子种子的抗真菌肽的报道。
采用包括四个色谱步骤的程序，从干谷子（Setaria italica（L.）Beauv.）种子中分离出一种分子量为 26.9 kDa 的抗真菌肽。该抗真菌肽被 CM-Sepharose、Affi-gel blue 凝胶和 Superdex 75 吸附。它通过 C（18）反相高效液相色谱进一步纯化，并提交肽质量指纹图谱分析。分离蛋白的 Mascot 肽质量指纹图谱未命中现有蛋白（得分>60），被证明是一种新型抗真菌肽。它以 1.3 µmol L(-1) 的 IC(50)抑制Alternaria alternate 的菌丝生长，并且对 Trichoderma viride、Botrytis cinerea 和 Fusarium oxysporum 也具有抗真菌活性。用 20 µg mL(-1) 的抗真菌蛋白孵育 48 h 后，交替链格孢霉菌的电镜观察显示真菌的超微结构发生了明显变化。
从谷子种子中分离出一种具有高效力的新型抗真菌肽。
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