Godfrey H P
Department of Pathology, New York Medical College, Basic Science, Valhalla.
Lymphokine Res. 1990 Fall;9(3):435-47.
T cell fibronectin (FN) is a product of antigen and mitogen activated human, murine and guinea pig T lymphocytes. Operationally and functionally, T cell FN is a lymphokine associated with delayed hypersensitivity. T cell FN acts at femtomolar concentrations to agglutinate mononuclear phagocytes and translocate monocytes and neutrophils through model extracellular matrices, and is 1.1 x 10(4) to 2.3 x 10(6) times more potent than other FN for these activities. It does not act on peripheral blood lymphocytes. Macrophage agglutination mediated by T cell FN requires cellular metabolism and depends on interactions between multiple classes of cell surface protein receptors and FN gelatin- and cell-binding domains. In contrast, translocation of cells through artificial matrices mediated by T cell FN is a biophysical process dependent on interactions between surface heparan sulfates on responding cells and FN amino-terminal heparin-binding and gelatin-binding domains. The correlation between the ability of cloned murine T cell lines to produce FN and their ability to transfer delayed hypersensitivity reactions suggests that secretion of T cell FN may be an important element in the initiation of these responses. The double activity of T cell FN could allow it to enhance influx of phagocytic effector cells and retain monocytes at tissue sites of T cell activation.
T细胞纤连蛋白（FN）是抗原和丝裂原激活的人、小鼠和豚鼠T淋巴细胞的产物。在操作和功能上，T细胞FN是一种与迟发型超敏反应相关的淋巴因子。T细胞FN在飞摩尔浓度下起作用，可凝集单核吞噬细胞，并使单核细胞和中性粒细胞通过模拟细胞外基质移位，并且在这些活性方面比其他FN强1.1×10⁴至2.3×10⁶倍。它对外周血淋巴细胞不起作用。由T细胞FN介导的巨噬细胞凝集需要细胞代谢，并依赖于多类细胞表面蛋白受体与FN明胶结合域和细胞结合域之间的相互作用。相比之下，由T细胞FN介导的细胞通过人工基质的移位是一个生物物理过程，依赖于反应细胞表面硫酸乙酰肝素与FN氨基末端肝素结合域和明胶结合域之间的相互作用。克隆的小鼠T细胞系产生FN的能力与其传递迟发型超敏反应的能力之间的相关性表明，T细胞FN的分泌可能是这些反应起始中的一个重要因素。T细胞FN的双重活性可能使其增强吞噬效应细胞的流入，并将单核细胞保留在T细胞激活的组织部位。
