Cho Se Youn, Yun Young Soo, Kim E Sle, Kim Moon Suk, Jin Hyoung-Joon
Department of Polymer Science and Engineering, Inha University, Incheon 402-751, Korea.
J Nanosci Nanotechnol. 2011 Jan;11(1):801-5. doi: 10.1166/jnn.2011.3181.
Multiwalled carbon nanotubes (MWCNTs) are considered to be the ideal reinforcements for biorelated applications on account of their remarkable structural, mechanical and thermal properties. However, before MWCNTs can be incorporated into new and existing biomedical devices, their toxicity and biocompatibility need to be investigated thoroughly. In this study, regenerated silk fibroin/MWCNT nanocomposite films were prepared using a solvent system with pre-dispersed MWCNTs. Their biocompatibility was examined in vitro using human bone marrow stem cells. Scanning electron microscopy and a WST-1 assay demonstrated that the silk fibroin/MWCN film supported BMSC attachment and growth over 7 days in culture similar to the silk fibroin only film.
多壁碳纳米管（MWCNTs）因其卓越的结构、机械和热性能而被认为是生物相关应用的理想增强材料。然而，在将多壁碳纳米管纳入新的和现有的生物医学设备之前，需要对其毒性和生物相容性进行彻底研究。在本研究中，使用含有预分散多壁碳纳米管的溶剂体系制备了再生丝素蛋白/多壁碳纳米管纳米复合薄膜。使用人骨髓干细胞在体外检测了它们的生物相容性。扫描电子显微镜和WST-1分析表明，丝素蛋白/多壁碳纳米管薄膜在培养7天内支持骨髓间充质干细胞附着和生长，与仅含丝素蛋白的薄膜相似。
