Johari Narges, Madaah Hosseini Hamid Reza, Samadikuchaksaraei Ali
Department of Materials Science and Engineering, Sharif University of Technology, Tehran 1458889694, Iran.
Department of Materials Science and Engineering, Sharif University of Technology, Tehran 1458889694, Iran.
Mater Sci Eng C Mater Biol Appl. 2018 Jan 1;82:265-276. doi: 10.1016/j.msec.2017.09.001. Epub 2017 Sep 4.
It is known that Fluoride ions strongly affect bone mineralization and formation. In the present study, the engineered bone tissue scaffolds are fabricated using silk fibroin (SF) and flouridated TiO nanoparticles. TiO nanoparticles are modified by fluoride ions, and different levels (0, 5, 10, 15 and 20wt%) of the fluoridated TiO nanoparticles (TiO-F) were subsequently added to the SF matrix through phase separation method to prepare silk fibroin/flouridated TiO nanocomposite scaffolds (SF/TiO-F). Phase structure, functional groups, morphology and mechanical properties of the obtained scaffolds were evaluated by X-ray diffraction method (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and compressive testing, respectively. In vitro degradation studies of scaffolds were performed by incubating the samples in phosphate buffered saline (PBS) at 37°C and pH7.4 for 30days. Additionally, the bioactivity of scaffolds was estimated in a simulated body fluid (SBF) buffered at 37°C and pH7.4 for 28days. Moreover, MTT assay was used to confirm the biocompatibility of the scaffolds using human like SaOS-2 osteoblast cell line for 1, 3 and 5days. The obtained results indicated that the mechanical properties of scaffolds have been improved by increasing the TiO-F amount up to 15wt%. However, a detrimental effect was observed by a further increase in the TiO-F content. The bioactivity of SF/TiO-F nanocomposite scaffolds was promoted by flouridation of TiO. Furthermore, cell cytotoxicity results demonstrated that the SF/TiO-F nanocomposite scaffolds are nontoxic to osteoblasts. The cell fixation results after 3days of incubation revealed that the cell attachment and spreading on SF/TiO-F nanocomposite scaffolds are improved with respect to SF/TiO nanocomposite scaffolds control sample.
已知氟离子会强烈影响骨矿化和形成。在本研究中，使用丝素蛋白（SF）和氟化钛纳米颗粒制备工程化骨组织支架。钛纳米颗粒用氟离子进行改性，随后通过相分离法将不同含量（0、5、10、15和20wt%）的氟化钛纳米颗粒（TiO-F）添加到SF基质中，以制备丝素蛋白/氟化钛纳米复合支架（SF/TiO-F）。分别通过X射线衍射法（XRD）、傅里叶变换红外光谱法（FTIR）、扫描电子显微镜（SEM）和压缩测试对所得支架的相结构、官能团、形态和力学性能进行评估。通过将样品在37°C和pH7.4的磷酸盐缓冲盐水（PBS）中孵育30天来进行支架的体外降解研究。此外，在37°C和pH7.4的模拟体液（SBF）中缓冲28天来评估支架的生物活性。此外，使用MTT法，以人源化的SaOS-2成骨细胞系培养1、3和5天来确认支架的生物相容性。所得结果表明，通过将TiO-F含量增加至15wt%，支架的力学性能得到了改善。然而，TiO-F含量的进一步增加则观察到有害影响。TiO的氟化促进了SF/TiO-F纳米复合支架的生物活性。此外，细胞毒性结果表明，SF/TiO-F纳米复合支架对成骨细胞无毒。孵育3天后的细胞固定结果显示，相对于SF/TiO纳米复合支架对照样品，SF/TiO-F纳米复合支架上的细胞附着和铺展得到了改善。
