Department of Medical Laboratory Science and Biotechnology, National Cheng Kung University, No. 1, University Road, Tainan 70101, Taiwan.
Future Med Chem. 2011 Mar;3(3):283-96. doi: 10.4155/fmc.10.290.
Carbohydrate-protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge.
We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates.
The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method.
This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses.
碳水化合物-蛋白质相互作用参与许多生物功能。对结合相互作用进行特征描述是一个长期存在的挑战。
我们开发了一种糖脂膜阵列来研究碳水化合物与凝集素、蛋白质和病毒的相互作用，其中包括 17 种凝集素、4 种抗体（针对不同的 Lewis 抗原）、一种重组 H5N1 血凝素和 5 种乙型流感临床分离株。
结果与之前的报道相当，表明我们开发的方法具有有效性和可靠性。
这种碳水化合物膜阵列代表了一种方便、可靠且低成本的方法，可以用于研究各种蛋白质的碳水化合物结合特征、高通量药物筛选以及流感病毒的糖结合监测。
