National Institute of Infectious Disease and Vaccinology, National Health Research Institutes, Tainan, Taiwan.
J Med Virol. 2012 Apr;84(4):679-85. doi: 10.1002/jmv.23219.
The carbohydrate binding specificities are different among avian and human influenza A viruses and may affect the tissue tropism and transmission of these viruses. The glycan binding biology for influenza B, however, has not been systematically characterized. Glycan binding specificities of influenza B viral isolates were analyzed and correlated to hemagglutinin (HA) genotypes and clinical manifestations. A newly developed solution glycan array was applied to characterize the receptor binding specificities of influenza B virus clinical isolates from 2001 to 2007 in Taiwan. Thirty oligosaccharides which include α-2,3 and α-2,6 linkage glycans were subjected to analysis. The glycan binding patterns of 53 influenza B isolates could be categorized into three groups and were well correlated to their HA genotypes. The Yamagata-like strains predominantly bound to α-2,6-linkage glycan (24:29, 83%) while Victoria-like strains preferentially bound to both α-2,3- and α-2,6-linkage glycans (13:24, 54%). A third group of viruses bound to sulfated glycans and these all belonged to Victoria-like strains. Based on the HA sequences, Asn-163, Glu-198, Ala-202, and Lys-203 were conserved among Victoria-like strains which may influence their carbohydrate recognition. The viruses bound to dual type glycans were more likely to be associated with the development of bronchopneumonia and gastrointestinal illness than those bound only to α-2,6 sialyl glycans (P < 0.05). Glycan binding analyses provide additional information to monitor the antigenic shift, tissue tropism, and transmission capability of influenza B viruses, and will contribute to virus surveillance and vaccine strain selection.
禽流感和人流感病毒的碳水化合物结合特异性不同，可能影响这些病毒的组织嗜性和传播。然而，乙型流感的糖结合生物学尚未得到系统表征。本研究分析了乙型流感病毒分离株的糖结合特异性，并将其与血凝素（HA）基因型和临床表现相关联。本研究应用新开发的溶液糖基芯片分析了 2001 年至 2007 年台湾地区乙型流感病毒临床分离株的受体结合特异性。共分析了 30 种寡糖，包括α-2,3 和α-2,6 连接的聚糖。53 株乙型流感分离株的糖结合模式可分为三组，与 HA 基因型密切相关。类似 Yamagata 的株主要结合α-2,6 连接的聚糖（24:29，83%），而类似 Victoria 的株则优先结合α-2,3-和α-2,6 连接的聚糖（13:24，54%）。第三组病毒结合硫酸化糖，这些都是类似 Victoria 的株。基于 HA 序列，类似 Victoria 的株在 Asn-163、Glu-198、Ala-202 和 Lys-203 处保守，这可能影响其碳水化合物识别。与仅结合α-2,6 唾液酸化聚糖的病毒相比，结合双类型聚糖的病毒更可能与支气管肺炎和胃肠道疾病的发生有关（P<0.05）。糖结合分析为监测乙型流感病毒的抗原转变、组织嗜性和传播能力提供了额外信息，并将有助于病毒监测和疫苗株选择。
