Rubin P M, Loreto E L S, Carareto C M A, Valente V L S
Programa de Pós-Graduação em Biodiversidade Animal, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Departamento de Biologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Genet Res (Camb). 2011 Jun;93(3):175-80. doi: 10.1017/S0016672310000625. Epub 2011 Mar 31.
The copia element is a retrotransposon that is hypothesized to have been horizontally transferred from Drosophila melanogaster to some populations of Drosophila willistoni in Florida. Here we have used PCR and Southern blots to screen for sequences similar to copia element in South American populations of D. willistoni, as well as in strains previously shown to be carriers of the element. We have not found the canonical copia element in any of these populations. Unlike the P element, which invaded the D. melanogaster genome from D. willistoni and quickly spread worldwide, the canonical copia element appears to have transferred in the opposite direction and has not spread. This may be explained by differences in the requirements for transposition and in the host control of transposition.
copia元件是一种反转录转座子，据推测它是从黑腹果蝇水平转移到佛罗里达州的一些威氏果蝇种群中的。在这里，我们使用聚合酶链式反应（PCR）和Southern杂交技术，对南美威氏果蝇种群以及先前已证明是该元件携带者的菌株中与copia元件相似的序列进行筛选。我们在这些种群中均未发现典型的copia元件。与从威氏果蝇侵入黑腹果蝇基因组并迅速在全球传播的P元件不同，典型的copia元件似乎是向相反方向转移的，且并未扩散。这可能是由于转座需求以及转座的宿主控制方面存在差异所致。
