Department of Medicine, Kidney Center, Tokyo Women's Medical University, Tokyo, Japan.
J Clin Pathol. 2011 Jun;64(6):504-9. doi: 10.1136/jcp.2010.087023. Epub 2011 Mar 30.
The aim of this study was to demonstrate expression of cell membrane invagination 'caveolae' in glomeruli and to correlate this with functional and structural characteristics of the human glomerular diseases.
The expression of caveolin-1 (Cav-1), which is the main component of caveolae, was examined in the glomeruli, and the relationship between Cav-1 expression and pathological and clinical findings was determined in 99 patients with glomerular disease and in 50 renal transplantation donors as controls.
Cav-1 was expressed very weakly in the controls, and the area of Cav-1 expression relative to the total glomerular area was 0.57±0.65%. However, the area of Cav-1 expression was significantly larger in each glomerular disease (IgA nephropathy, 1.05±1.36%, p<0.05; crescent glomerulonephritis, 1.86±1.19%, p<0.001; minimal change disease, 2.38±1.24%, p<0.001; focal segmental glomerulosclerosis, 2.88±2.05%, p<0.01; membranous nephritis, 4.27±2.95%, p<0.001; membranoproliferative glomerulonephritis, 4.49±3.15%, p<0.001; and diabetic nephropathy, 2.45±1.52%, p<0.001; compared with the controls. Cav-1 expression was significantly decreased in glomerular disease treated with steroids. Co-localisation of Cav-1 and the endothelial marker 'pathologische anatomie leiden-endothelium' was prominent in an immunofluorescence study, and caveolae on the glomerular endothelial cells were observed in electron microscopy.
The expression of Cav-1 was significantly increased in the glomeruli of patients with glomerular disease, and it was related to urinary albumin excretion. Cav-1 expression and caveolae were observed in glomerular endothelial cells. It is hypothesised that they play a role in the recovery phase of capillary injury or endocytosis of albumin into endothelial cells. Basic research should be performed to elucidate the role played by Cav-1 and caveolae.
本研究旨在展示细胞膜内陷“小窝”在肾小球中的表达，并将其与人类肾小球疾病的功能和结构特征相关联。
检测了小窝主要成分 caveolin-1（Cav-1）在肾小球中的表达，并在 99 例肾小球疾病患者和 50 例肾移植供体中确定了 Cav-1 表达与病理和临床发现之间的关系。
对照组中 Cav-1 的表达非常弱，Cav-1 表达面积相对于肾小球总面积为 0.57±0.65%。然而，每种肾小球疾病的 Cav-1 表达面积均显著增大（IgA 肾病为 1.05±1.36%，p<0.05；新月体性肾小球肾炎为 1.86±1.19%，p<0.001；微小病变性肾病为 2.38±1.24%，p<0.001；局灶节段性肾小球硬化症为 2.88±2.05%，p<0.01；膜性肾病为 4.27±2.95%，p<0.001；膜增殖性肾小球肾炎为 4.49±3.15%，p<0.001；糖尿病肾病为 2.45±1.52%，p<0.001；与对照组相比。用类固醇治疗的肾小球疾病中 Cav-1 的表达显著降低。免疫荧光研究中 Cav-1 与内皮标志物“pathologische anatomie leiden-endothelium”的共定位明显，电镜下观察到肾小球内皮细胞上的小窝。
肾小球疾病患者肾小球中 Cav-1 的表达显著增加，与尿白蛋白排泄量有关。在肾小球内皮细胞中观察到 Cav-1 表达和小窝。推测它们在毛细血管损伤的恢复阶段或白蛋白内吞进入内皮细胞中发挥作用。应进行基础研究以阐明 Cav-1 和小窝的作用。
