Haematology Department, University Hospital's Bristol NHS Foundation Trust, Bristol, UK.
J Clin Pathol. 2011 Jul;64(7):561-3. doi: 10.1136/jcp.2010.085183. Epub 2011 Mar 30.
Multicolour flow cytometry in haematology has developed considerably in recent years. The ability to analyse eight or more colours of fluorescence on millions of cells in a matter of minutes has enabled the provision of rapid and reliable measures of minimal residual disease for clinicians. The use of multicolour analysis has also enabled more specific characterisation of presenting leukaemias and lymphomas. However, there has not been a concomitant increase in the knowledge and experience of the flow cytometrists to deal with certain problems associated with this more complex analysis.
近年来,血液学中的多色流式细胞术有了显著的发展。能够在数分钟内对上百万个细胞进行多达八种或更多种颜色的荧光分析,为临床医生提供了快速可靠的微小残留病检测手段。多色分析的应用也使得对白血病和淋巴瘤的表现特征有了更具针对性的描述。然而,流式细胞仪技术人员在处理与这种更复杂分析相关的某些问题方面,并没有相应地增加知识和经验。