Ebert E C
Department of Medicine, UMDNJ-Robert Wood Johnson Medical School, New Brunswick 08903-0019.
Clin Exp Immunol. 1990 Oct;82(1):81-5. doi: 10.1111/j.1365-2249.1990.tb05407.x.
Human intraepithelial lymphocytes (IEL) proliferate minimally in response to phytohaemagglutinin (PHA), but produce as much interleukin-2 (IL-2) as do peripheral blood lymphocytes (PBL). The addition of sheep erythrocytes during activation of IEL with PHA markedly augments both T cell functions. This study evaluates the ability of IEL to produce interferon-gamma (IFN-gamma) and to develop suppressor and cytotoxic activities when stimulated with mitogens in the presence or absence of sheep erythrocytes. PHA-activated IEL produced as much IFN-gamma as did PHA-activated peripheral blood CD8+ T lymphocytes. IEL activated by concanavalin A (Con A) demonstrated less suppressor activity directed against T cell proliferation than did Con A-activated peripheral blood CD8+ T lymphocytes. IEL generated less mitogen-induced cellular cytotoxicity and lymphokine-activated killer cell activity than did peripheral blood CD8+ T lymphocytes. The addition of sheep erythrocyte lysates during mitogen stimulation of IEL markedly enhanced their proliferation and lymphokine production but did not affect their suppressor or cytotoxic activities.
人上皮内淋巴细胞（IEL）对植物血凝素（PHA）的反应增殖极少，但产生的白细胞介素-2（IL-2）与外周血淋巴细胞（PBL）一样多。在用PHA激活IEL的过程中添加绵羊红细胞可显著增强这两种T细胞功能。本研究评估了在有或没有绵羊红细胞存在的情况下，IEL在受到丝裂原刺激时产生干扰素-γ（IFN-γ）以及产生抑制和细胞毒性活性的能力。PHA激活的IEL产生的IFN-γ与PHA激活的外周血CD8+ T淋巴细胞一样多。与伴刀豆球蛋白A（Con A）激活的外周血CD8+ T淋巴细胞相比，Con A激活的IEL对T细胞增殖的抑制活性较低。与外周血CD8+ T淋巴细胞相比，IEL产生的丝裂原诱导的细胞毒性和淋巴因子激活的杀伤细胞活性较低。在对IEL进行丝裂原刺激时添加绵羊红细胞裂解物可显著增强其增殖和淋巴因子产生，但不影响其抑制或细胞毒性活性。
