Dillon S B, Dalton B J, MacDonald T T
Cell Immunol. 1986 Dec;103(2):326-38. doi: 10.1016/0008-8749(86)90093-6.
Although most intraepithelial lymphocytes (IEL) in mouse small intestine bear surface markers classically associated with T lymphocytes, the T-cell nature of these cells remains controversial. In the present study IEL from normal mice, or from mice infected with the gut nematode Trichinella spiralis, were therefore tested for their ability to produce T-cell-derived lymphokines in response to in vitro stimulation with concanavalin A (Con A) or with specific worm antigens. The data show that Con A-stimulated IEL produce minimal amounts of IL-2, and intermediate levels of IFN-gamma and IL-3 in comparison to the levels produced by spleen T cells. The FDC-P2 cell line, which proliferates in response to both IL-3 and GM-CSF, was identified as the most sensitive and reproducible indicator of lymphokine activity in supernatants from mitogen-stimulated IEL from normal mice. IEL isolated from mice infected with T. spiralis also produced high levels of FDC-P2 growth factors when challenged in vitro with Trichinella-derived antigens; however, normal IEL did not respond to this stimulus. The data thus provide evidence that antigen-sensitive T cells can arise in (or migrate to) the gut epithelium during gut infection.
尽管小鼠小肠中的大多数上皮内淋巴细胞（IEL）带有通常与T淋巴细胞相关的表面标志物，但这些细胞的T细胞性质仍存在争议。因此，在本研究中，对来自正常小鼠或感染肠道线虫旋毛虫的小鼠的IEL进行了测试，以检测它们在体外用凝集素A（Con A）或特定蠕虫抗原刺激后产生T细胞衍生淋巴因子的能力。数据显示，与脾脏T细胞产生的水平相比，Con A刺激的IEL产生的IL-2量极少，IFN-γ和IL-3水平处于中等。FDC-P2细胞系可响应IL-3和GM-CSF而增殖，被确定为正常小鼠有丝分裂原刺激的IEL上清液中淋巴因子活性最敏感且可重复的指标。从感染旋毛虫的小鼠中分离出的IEL在用旋毛虫衍生抗原进行体外刺激时，也会产生高水平的FDC-P2生长因子；然而，正常IEL对这种刺激无反应。因此，数据提供了证据表明，在肠道感染期间，抗原敏感的T细胞可在肠道上皮中产生（或迁移至肠道上皮）。
