Lomnitzer R, Phillips R, Rabson A R
Cell Immunol. 1984 Jul;86(2):362-70. doi: 10.1016/0008-8749(84)90391-5.
When PHA-activated normal responder cells (R cells) were cocultured with mononuclear cells (MN cells) which had been preincubated for 48 hr in medium alone (C cells) an enhanced proliferative response was observed. This enhancement was only obtained when the R cells were cultured with allogeneic C cells or when PHA was in the cocultures for the entire culture period. This effect was due to greater production of interleukin 2 (IL-2) by irradiated C cells in the presence of allogeneic or mitogenic stimulation. Con A-treated mononuclear cells (S cells) cultured with PHA-activated allogeneic or autologous responder cells showed reduced [3H]thymidine incorporation and IL-2 production as compared to activated R cells alone. Glutaraldehyde-treated S cells (which retained the ability to absorb IL-2) did not affect the proliferative response or IL-2 production by the R cells, indicating that passive absorption of IL-2 was not entirely responsible for suppression induced by S cells. S cells, pretreated with IL-2, still inhibited R-cell activity. These results show that Con A-treated MN cells suppressed or prevented [3H]thymidine incorporation by actively inhibiting IL-2 production.
当PHA激活的正常反应细胞（R细胞）与已在单独培养基中预孵育48小时的单核细胞（MN细胞，即C细胞）共培养时，观察到增殖反应增强。只有当R细胞与同种异体C细胞共培养，或者在整个培养期间PHA存在于共培养体系中时，才会出现这种增强。这种效应是由于在同种异体或促有丝分裂刺激存在下，受辐照的C细胞产生了更多的白细胞介素2（IL-2）。与单独的活化R细胞相比，用Con A处理的单核细胞（S细胞）与PHA激活的同种异体或自体反应细胞共培养时，[3H]胸苷掺入量和IL-2产生量降低。经戊二醛处理的S细胞（保留吸收IL-2的能力）不影响R细胞的增殖反应或IL-2产生，这表明IL-2的被动吸收并非S细胞诱导抑制的全部原因。用IL-2预处理的S细胞仍然抑制R细胞活性。这些结果表明，Con A处理的MN细胞通过积极抑制IL-2产生来抑制或阻止[3H]胸苷掺入。
