Rubakhin Stanislav S, Sweedler Jonathan V
Department of Chemistry and Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Methods Mol Biol. 2010;656:21-49. doi: 10.1007/978-1-60761-746-4_2.
Mass spectrometry imaging (MSI), a rapidly growing subfield of chemical imaging, employs mass spectrometry (MS) technologies to create single- and multi-dimensional localization maps for a variety of atoms and molecules. Complimentary to other imaging approaches, MSI provides high chemical specificity and broad analyte coverage. This powerful analytical toolset is capable of measuring the distribution of many classes of inorganics, metabolites, proteins, and pharmaceuticals in chemically and structurally complex biological specimens in vivo, in vitro, and in situ. The MSI approaches highlighted in this Methods in Molecular Biology volume provide flexibility of detection, characterization, and identification of multiple known and unknown analytes. The goal of this chapter is to introduce investigators who may be unfamiliar with MS to the basic principles of the mass spectrometric approaches as used in MSI. In addition to guidelines for choosing the most suitable MSI method for specific investigations, cross-references are provided to the chapters in this volume that describe the appropriate experimental protocols.
质谱成像（MSI）是化学成像领域中一个快速发展的子领域，它采用质谱（MS）技术为各种原子和分子创建一维和多维定位图。作为其他成像方法的补充，MSI具有高化学特异性和广泛的分析物覆盖范围。这种强大的分析工具集能够在体内、体外和原位测量化学和结构复杂的生物样本中多种无机化合物、代谢物、蛋白质和药物的分布。本《分子生物学方法》卷中重点介绍的MSI方法提供了检测、表征和鉴定多种已知和未知分析物的灵活性。本章的目的是向可能不熟悉MS的研究人员介绍MSI中使用的质谱方法的基本原理。除了为特定研究选择最合适的MSI方法的指南外，还提供了指向本卷中描述适当实验方案的章节的交叉引用。
