Institute of Stem Cell Research, Helmholtz Zentrum Munich-German Research Center for Environmental Health GmbH, Neuherberg, Germany.
Nat Methods. 2011 Apr;8(4 Suppl):S30-5. doi: 10.1038/nmeth.1577. Epub 2011 Mar 30.
Continuous long-term single-cell observation provides insight into the molecular control of cell fate. This is particularly important for rare and heterogeneous populations of cells, such as mammalian stem cells. The current lack of usable off-the-shelf hardware and software for such experiments makes their implementation technically challenging. Here I discuss the need for continuous single-cell quantification to understand molecular cell fate control as well as organizational and technical solutions for long-term imaging and tracking of stem cells.
连续的长期单细胞观察为细胞命运的分子控制提供了深入的了解。对于哺乳动物干细胞等稀有和异质细胞群体来说，这一点尤为重要。目前，此类实验缺乏可用的现成硬件和软件，这使得它们的实施具有技术挑战性。在这里，我讨论了连续单细胞定量分析对于理解分子细胞命运控制的必要性，以及用于长期成像和跟踪干细胞的组织和技术解决方案。
