Bondy G S, Pestka J J
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824-1224.
Toxicol Appl Pharmacol. 1991 May;108(3):520-30. doi: 10.1016/0041-008x(91)90098-y.
The effects of 8 weeks of dietary exposure to the fungal toxin vomitoxin (25 ppm) on the kinetics of in vitro immunoglobulin (Ig) production and appearance of IgA-secreting cells in lymphocyte culture were assessed in the B6C3F1 mouse. The feeding regimen resulted in an IgA:IgG serum ratio of 2.4 compared to 0.4 in controls indicating that there was dysregulation of IgA production in the systemic compartment. Prior toxin feeding had no effect on viability of Peyer's patch (PP) or splenic lymphocyte cultures. IgA production, as determined by enzyme-linked immunosorbent assay, was significantly greater in treatment PP and splenic lymphocytes cultured for 2-11 days than in corresponding controls. Similar trends were found for IgG production in PP cultures although levels were much lower. There were 1.7 and 2.0 times more IgA-producing cells, as measured by the ELISPOT assay, in freshly prepared PP and splenic lymphocytes from treatment mice compared to control mice, respectively. In contrast, after 2 days there were 10.9, 3.2, and 12.4 times more IgA-secreting cells in concanavalin A (Con A), LPS, and unstimulated treatment PP cultures, respectively, and 4.0, 2.0, and 3.5 times times more IgA-secreting cells in 2-day treatment spleen cultures, respectively. Both IgA and IgG secretion in Con A-stimulated cultures were significantly greater when treatment T cells and control B cells were combined than when control T cells and control B cells were combined. Increased Ig secretion attributable to T cell effects was not observed in LPS-stimulated or unstimulated PP reconstitution cultures or in spleen reconstituted cultures with and without mitogen. The results provide evidence that dietary vomitoxin enhances terminal differentiation of IgA secreting cells in PP. This and resultant migration of IgA secreting cells into the systemic compartment favor a shift from IgG to IgA as the primary serum isotype.
在B6C3F1小鼠中评估了8周饮食暴露于真菌毒素呕吐毒素（25 ppm）对体外免疫球蛋白（Ig）产生动力学以及淋巴细胞培养中分泌IgA细胞出现情况的影响。喂养方案导致血清中IgA:IgG比例为2.4，而对照组为0.4，这表明全身区室中IgA产生失调。先前的毒素喂养对派尔集合淋巴结（PP）或脾淋巴细胞培养物的活力没有影响。通过酶联免疫吸附测定法测定，处理组PP和培养2 - 11天的脾淋巴细胞中的IgA产生明显高于相应对照组。PP培养物中IgG产生也有类似趋势，尽管水平低得多。通过ELISPOT测定法测量，与对照小鼠相比，处理组小鼠新鲜制备的PP和脾淋巴细胞中产生IgA的细胞分别多1.7倍和2.0倍。相比之下，2天后，伴刀豆球蛋白A（Con A）、脂多糖（LPS）和未刺激的处理组PP培养物中分泌IgA的细胞分别多10.9倍、3.2倍和12.4倍，处理组2天的脾培养物中分泌IgA的细胞分别多4.0倍、2.0倍和3.5倍。当处理组T细胞和对照组B细胞组合时，Con A刺激培养物中的IgA和IgG分泌均显著高于对照组T细胞和对照组B细胞组合时。在LPS刺激或未刺激的PP重建培养物或有丝分裂原存在或不存在的脾重建培养物中，未观察到归因于T细胞效应的Ig分泌增加。结果提供了证据表明饮食中的呕吐毒素增强了PP中分泌IgA细胞向终末分化。这以及由此导致的分泌IgA细胞迁移到全身区室有利于从IgG向IgA作为主要血清同种型的转变。
