Kataoka T, Nakamura M, Iwaguchi T
Jpn J Cancer Res. 1985 Oct;76(10):995-1002.
Spleen cells of mice inoculated intravenously (iv) with concanavalin A (Con A) suppressed the in vitro normal spleen cell blastogenesis. The suppressor cells, as determined by measuring the inhibition of in vitro T cell blastogenesis by Con A, were resistant to X-irradiation. They lacked surface Ig and were abrogated by treatment with alpha Thy 1.2 antibody and complement, but not with complement alone, indicating that these suppressor cells are not B cells but T cells. Inoculation of 5-fluorouracil (5-FU) inhibited the induction and/or activity of these suppressor cells, whereas that of cyclophosphamide (CY), adriamycin, vincristine, or 3-[(4-amino-2-methyl-5-pyrimidinyl)-methyl]-1-(2-chloroethyl)-1-nitro sou rea (ACNU) did not at any interval tested. Since two other antimetabolic agents, 6-mercaptopurine and 2'-deoxyguanosine, were also effective in inhibiting these suppressor T cells, it was supposed that Con A-induced suppressor T cells are metabolically more active than the other T subpopulations. The findings that CY and ACNU decreased the number of T cells as much as or more than 5-FU did, but that they did not inhibit these suppressor T cells, suggests that 5-FU more or less selectively inhibited this suppressor T-cell subpopulation.
静脉注射伴刀豆球蛋白A（Con A）的小鼠脾细胞抑制了体外正常脾细胞的增殖。通过测量Con A对体外T细胞增殖的抑制作用来确定的抑制细胞对X射线照射具有抗性。它们缺乏表面免疫球蛋白，用抗αThy 1.2抗体和补体处理可消除其作用，但单独用补体处理则不能，这表明这些抑制细胞不是B细胞而是T细胞。接种5-氟尿嘧啶（5-FU）可抑制这些抑制细胞的诱导和/或活性，而环磷酰胺（CY）、阿霉素、长春新碱或3-[(4-氨基-2-甲基-5-嘧啶基)-甲基]-1-(2-氯乙基)-1-硝基脲（ACNU）在任何测试时间间隔均无此作用。由于另外两种抗代谢剂6-巯基嘌呤和2'-脱氧鸟苷也能有效抑制这些抑制性T细胞，因此推测Con A诱导的抑制性T细胞在代谢上比其他T细胞亚群更活跃。CY和ACNU使T细胞数量减少的程度与5-FU相同或更多，但它们并不抑制这些抑制性T细胞，这一发现表明5-FU或多或少地选择性抑制了这个抑制性T细胞亚群。
