Department of Chemical Engineering, McGill University, Montreal, Quebec H3A 2B2, Canada.
Langmuir. 2011 May 3;27(9):5472-80. doi: 10.1021/la200102z. Epub 2011 Mar 31.
The rise of antibiotic-resistant bacteria has directed substantial attention toward the use of bacteriophages as a means to control bacterial populations. It has been proposed that bacteriophages can be applied as a coating on surfaces in healthcare settings or on indwelling medical devices to create an antimicrobial surface. In this study, antimicrobial model surfaces functionalized with five different types of bacteriophage were prepared and characterized with X-ray photoelectron spectroscopy and atomic force microscopy. The bacterial capture efficiency of these functionalized surfaces was studied for two common bacteria, Escherichia coli and Salmonella typhimurium. Binding of the phages to a solid surface affected their biofunctionality as expressed by the capture efficiency and rate of host membrane disruption. Moreover, the size and shape of the bacteriophage and positioning of its specific binding proteins significantly affected its bacterial capture capability in the immobilized state. Symmetric bacteriophages were found to be a better choice for antibacterial surfaces compared to more asymmetric tailed bacteriophages. Immobilized phages were found to disrupt the membranes of attached bacteria and are thus proposed as a candidate for antimicrobial surfaces.
抗生素耐药菌的出现引起了人们对噬菌体作为控制细菌种群的一种手段的广泛关注。有人提出，噬菌体可以作为涂层应用于医疗保健环境中的表面或留置的医疗设备上，以创建抗菌表面。在这项研究中，制备了功能化有五种不同类型噬菌体的抗菌模型表面，并通过 X 射线光电子能谱和原子力显微镜对其进行了表征。研究了这些功能化表面对两种常见细菌（大肠杆菌和鼠伤寒沙门氏菌）的细菌捕获效率。噬菌体与固体表面的结合影响了它们的生物功能，表现为捕获效率和宿主膜破坏的速率。此外，噬菌体的大小和形状及其特异性结合蛋白的定位显著影响了其在固定状态下的细菌捕获能力。与具有更多不对称尾部的噬菌体相比，对称噬菌体被认为是抗菌表面的更好选择。固定化噬菌体被发现能够破坏附着细菌的膜，因此被提议作为抗菌表面的候选物。
