Ibrahim Iddrisu, Ayariga Joseph Atia, Xu Junhuan, Adebanjo Ayomide, Robertson Boakai K, Samuel-Foo Michelle, Ajayi Olufemi S
The Microbiology Program, College of Science, Technology, Engineering, and Mathematics (C-STEM), Alabama State University, Montgomery, AL, United States.
The Industrial Hemp Program, College of Science, Technology, Engineering, and Mathematics (C-STEM), Alabama State University, Montgomery, AL, United States.
Front Med (Lausanne). 2023 Mar 7;10:1075698. doi: 10.3389/fmed.2023.1075698. eCollection 2023.
The rise of antimicrobial resistance is a global public health crisis that threatens the effective control and prevention of infections. Due to the emergence of pandrug-resistant bacteria, most antibiotics have lost their efficacy. Bacteriophages or their components are known to target bacterial cell walls, cell membranes, and lipopolysaccharides (LPS) and hydrolyze them. Bacteriophages being the natural predators of pathogenic bacteria, are inevitably categorized as "human friends", thus fulfilling the adage that "the enemy of my enemy is my friend". Leveraging on their lethal capabilities against pathogenic bacteria, researchers are searching for more ways to overcome the current antibiotic resistance challenge. In this study, we expressed and purified epsilon 34 phage tailspike protein (E34 TSP) from the E34 TSP gene, then assessed the ability of this bacteriophage protein in the killing of two CBD-resistant strains of spp. We also assessed the ability of the tailspike protein to cause bacteria membrane disruption, and dehydrogenase depletion. We observed that the combined treatment of CBD-resistant strains of with CBD and E34 TSP showed poor killing ability whereas the monotreatment with E34 TSP showed considerably higher killing efficiency. This study demonstrates that the inhibition of the bacteria by E34 TSP was due in part to membrane disruption, and dehydrogenase inactivation by the protein. The results of this work provides an interesting background to highlight the crucial role phage protein such as E34 TSP could play in pathogenic bacterial control.
抗菌药物耐药性的出现是一场全球公共卫生危机，威胁着感染的有效控制和预防。由于泛耐药菌的出现，大多数抗生素已失去疗效。已知噬菌体或其成分可靶向细菌细胞壁、细胞膜和脂多糖（LPS）并将其水解。噬菌体作为病原菌的天然捕食者，不可避免地被归类为“人类的朋友”，从而应了那句“敌人的敌人就是我的朋友”的格言。利用它们对病原菌的致命能力，研究人员正在寻找更多方法来克服当前的抗生素耐药性挑战。在本研究中，我们从E34 TSP基因中表达并纯化了ε34噬菌体尾刺蛋白（E34 TSP），然后评估了这种噬菌体蛋白对两种 spp. 的CBD耐药菌株的杀伤能力。我们还评估了尾刺蛋白导致细菌膜破坏和脱氢酶消耗的能力。我们观察到，用CBD和E34 TSP联合处理 的CBD耐药菌株显示出较差的杀伤能力，而单独用E34 TSP处理则显示出相当高的杀伤效率。这项研究表明，E34 TSP对细菌的抑制部分归因于膜破坏以及该蛋白导致的脱氢酶失活。这项工作的结果提供了一个有趣的背景，以突出诸如E34 TSP之类的噬菌体蛋白在病原菌控制中可能发挥的关键作用。
