Empire State College, SUNY , P.O. Box 908, Fort Drum, New York 13602-0908, USA.
ACS Appl Mater Interfaces. 2011 May;3(5):1620-3. doi: 10.1021/am200165m. Epub 2011 Apr 12.
Biomarkers characteristic of liver injury, alanine transaminase and lactate dehydrogenase, were processed by an enzyme-based system functioning as a logic AND gate. The NAD+ output signal produced by the system upon its activation in the presence of both biomarkers was then biocatalytically converted to a decrease in pH. The acidic pH value biocatalytically produced by the system as a response to the biomarkers triggered the restructuring of a polymer-modified electrode interface. This allowed a soluble redox species to approach the electrode surface, thus switching the electrochemical reaction ON. The redox transformations activated by the biochemical signals resulted in an amplification of signals. This system represents the first example of an integrated sensing-actuating chemical device with the implemented AND Boolean logic for processing natural biomarkers at their physiologically relevant concentrations.
生物标志物特征的肝损伤，丙氨酸氨基转移酶和乳酸脱氢酶，被酶处理的系统功能作为一个逻辑与门。该 NAD + 输出信号产生的系统激活时，在存在两种生物标志物，然后生物催化转化为减少 pH 值。在酸性 pH 值生物催化产生的系统作为一个回应生物标志物引发了重组聚合物修饰电极界面。这使得一种可溶性氧化还原物种接近电极表面，从而打开电化学反应。氧化还原转化激活的生化信号导致信号的放大。该系统代表了第一个集成传感-执行化学装置的例子，具有实现的 AND 布尔逻辑，用于处理生理相关浓度下的天然生物标志物。
