Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
Talanta. 2011 Jul 15;85(1):800-3. doi: 10.1016/j.talanta.2011.03.034. Epub 2011 Apr 1.
A cooperative effect of two biomarkers, α-amylase and lactate dehydrogenase, was used to analyze radiation-caused tissue damage in vitro in model solutions of human serum. The analytical system was based on the recently emerged biocomputing concept applying biocatalytic cascades for logic processing of biochemical input signals. The studied system resembled a Boolean NAND logic gate in which the change of the optical output signal from a high level (logic value 1) to a low level (logic value 0) confirmed the presence of both biomarkers at pathological concentrations (1,1 input signals), thus yielding the conclusion about radiation tissue damage. The system operates in a digital YES/NO format as an alert-type biosensor with a built-in Boolean logic.
两种生物标志物（α-淀粉酶和乳酸脱氢酶）的协同作用被用于分析人血清模型溶液中体外辐射引起的组织损伤。该分析系统基于最近出现的生物计算概念，即应用生物催化级联对生化输入信号进行逻辑处理。所研究的系统类似于布尔 NAND 逻辑门，其中光学输出信号从高水平（逻辑值 1）变为低水平（逻辑值 0）的变化证实了两种生物标志物均处于病理浓度（1,1 输入信号），从而得出关于辐射组织损伤的结论。该系统以数字 YES/NO 格式作为具有内置布尔逻辑的警报型生物传感器运行。
