Department of Chemistry, Stanford University, Stanford, California 94305, United States.
Langmuir. 2011 May 3;27(9):5492-7. doi: 10.1021/la200234h. Epub 2011 Mar 31.
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe, R. D.; Chan, Y-H. M.; Ganesan, P. V.; Boxer, S. G. J. Struct. Biol.2009, 168, 190-9). When mobile DNA tethers are used, the tethered bilayer patches become unstable, while they are stable if the tethers are fixed on the surface. Because the mobile tethers between a patch and a supported lipid bilayer offer a particularly interesting architecture for studying the dynamics of membrane-membrane interactions, we have investigated the sources of instability, focusing on membrane composition. The most stable patches were made with a mixture of saturated lipids and cholesterol, suggesting an important role for membrane stiffness. Other factors such as the effect of tether length, lateral mobility, and patch membrane edge were also investigated. On the basis of these results, a model for the mechanism of patch destruction is developed.
我们最近引入了两种方法，通过 DNA 杂交将平面脂质双层（作为膜片）连接到支持脂质双层或 DNA 功能化表面：（Chung，M.；Lowe，R. D.；Chan，Y-H. M.；Ganesan，P. V.；Boxer，S. G. J. Struct. Biol.2009，168，190-9）。当使用可移动的 DNA 系链时，系链的双层片变得不稳定，而如果系链固定在表面上则稳定。由于片和支持脂质双层之间的移动系链提供了用于研究膜-膜相互作用动力学的特别有趣的结构，我们研究了不稳定性的来源，重点关注膜组成。最稳定的片由饱和脂质和胆固醇的混合物制成，表明膜刚度起着重要作用。还研究了其他因素，如系链长度、横向流动性和片膜边缘的影响。基于这些结果，提出了片破坏的机制模型。
